Anti-Smac/Diablo抗体(ab8114)
Key features and details
- Rabbit polyclonal to Smac/Diablo
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Smac/Diablo抗体
参阅全部 Smac/Diablo 一抗 -
描述
兔多克隆抗体to Smac/Diablo -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Peptide (
EERAESEQEAYLRED
)corresponding to amino acids 225 to 239 of humanSmac/DIABLO (1). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Smac Antibody is affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
|
|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa. |
靶标
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功能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
组织特异性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
结构域
The mature N-terminus mediates interaction with XIAP. -
细胞定位
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
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别名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
图片
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Western blot - Anti-Smac/Diablo antibody (ab8114)All lanes : Anti-Smac/Diablo antibody (ab8114) at 1 µg/ml
Lane 1 : human heart tissue lysate with absence of blocking peptide
Lane 2 : human heart tissue lysate with Smac / Diablo peptide (ab8380)
Predicted band size: 25 kDa
Observed band size: 25 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody (ab8114)Immunohistochemical analysis of paraffin-embedded human ovary tissue with ab8114 at 5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-Smac/Diablo antibody (ab8114)All lanes : Anti-Smac / Diablo antibody (ab8114) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.
Loading: 15 μg of lysates per lane.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution. -
Western blot - Anti-Smac/Diablo antibody (ab8114)Anti-Smac/Diablo antibody (ab8114) at 1 µg/ml + NIH3T3 cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 25 kDa
数据表及文件
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Datasheet download
文献 (9)
ab8114 被引用在 9 文献中.
- Wang X et al. Cytosolic adaptation to mitochondria-induced proteostatic stress causes progressive muscle wasting. iScience 25:103715 (2022). PubMed: 35072007
- Mayr SJ et al. Alternative splicing of the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1) uncovers a novel mitochondrial protein maturation mechanism. J Biol Chem 295:3029-3039 (2020). PubMed: 31996372
- Chen W et al. Gemcitabine combined with an engineered oncolytic vaccinia virus exhibits a synergistic suppressive effect on the tumor growth of pancreatic cancer. Oncol Rep 41:67-76 (2019). PubMed: 30365143
- Cheng CY et al. Ferulic Acid Exerts Anti-apoptotic Effects against Ischemic Injury by Activating HSP70/Bcl-2- and HSP70/Autophagy-Mediated Signaling after Permanent Focal Cerebral Ischemia in Rats. Am J Chin Med 47:39-61 (2019). PubMed: 30612456
- Liu Y et al. Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol. Mol Biol Cell 30:1272-1284 (2019). PubMed: 30893019
- Tan S et al. Sodium Selenite Accentuates the Therapeutic Effect of Adriamycin Prodrug (PADM) against Gastric Cancer. Biomed Res Int 2019:2035682 (2019). PubMed: 31737654
- Cheng CY et al. Electroacupuncture at different frequencies (5Hz and 25Hz) ameliorates cerebral ischemia-reperfusion injury in rats: possible involvement of p38 MAPK-mediated anti-apoptotic signaling pathways. BMC Complement Altern Med 15:241 (2015). WB ; Rat . PubMed: 26187498
- Martin B et al. GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging. Front Endocrinol (Lausanne) 6:191 (2015). PubMed: 26834700
- Bannwarth S et al. A mitochondrial origin for frontotemporal dementia and amyotrophic lateral sclerosis through CHCHD10 involvement. Brain 137:2329-45 (2014). PubMed: 24934289
