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AB86594

Anti-Sly1抗体

Anti-Sly1 antibody

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(2 Publications)

Rabbit Polyclonal Sly1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SCFD1 aa 300-350.

查看别名

C14orf163, KIAA0917, STXBP1L2, FKSG23, SCFD1, Sec1 family domain-containing protein 1, SLY1 homolog, Syntaxin-binding protein 1-like 2, Sly1p

2 Images
Immunoprecipitation - Anti-Sly1 antibody (AB86594)
  • IP

Unknown

Immunoprecipitation - Anti-Sly1 antibody (AB86594)

1mg whole cell lysate from Hela cells was immunoprecipited using 3ug ab86594. 20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
Detection : chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Sly1 antibody (ab86594)

Predicted band size: 72 kDa

false

Western blot - Anti-Sly1 antibody (AB86594)
  • WB

Unknown

Western blot - Anti-Sly1 antibody (AB86594)

All lanes:

Western blot - Anti-Sly1 antibody (ab86594) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 72 kDa

false

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human SCFD1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q8WVM8

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搭配使用产品

Primary Antibodies

AB155510

Anti-BLZF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-BLZF1 antibody (AB155510)

  • 0
  • 0
Primary Antibodies

AB134952

Anti-ACBD3 antibody [EPR8623]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ACBD3 antibody [EPR8623] (AB134952)

  • 5
  • 1
Primary Antibodies

AB118792

Anti-EXOC7 antibody

Immunoprecipitation - Anti-EXOC7 antibody (AB118792)

  • 0
  • 0
Primary Antibodies

AB229213

Anti-SMC4 antibody - N-terminal

Immunoprecipitation - Anti-SMC4 antibody - N-terminal (AB229213)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Sly1 also known as suppressor of Ypt1 homolog 1 functions as a protein involved in vesicle fusion. It has a molecular mass of approximately 67 kDa and serves as an important element in vesicular trafficking processes. Expressed in various tissues Sly1 primarily resides in the cytoplasm and the Golgi apparatus. It facilitates the fusion of transport vesicles to compartment membranes by binding to SNARE proteins therefore playing a role in maintaining normal cellular transport operations.
Biological function summary

Sly1 influences cellular transport by being part of the SM (Sec1/Munc18) protein family. It regulates SNARE complex assembly ensuring accurate vesicle docking and fusion events. Sly1 interacts with different t-SNAREs such as Syntaxin 5 modulating their functions and energy coupling during vesicle fusion. Proper action of Sly1 is necessary for maintaining cellular organization and intracellular communication influencing the efficiency of endocytotic and exocytotic pathways.

Pathways

Sly1 participates actively within the vesicle transport mechanisms linked by SNARE proteins. It plays a fundamental role in the secretory pathway affecting protein and lipid trafficking. Interacting with proteins such as Ypt1 a small GTPase Sly1 targets vesicle transport to the correct membranes for fusion. These interactions are important for the functionality of Golgi-mediated vesicle transport and signal transductions as well as for maintaining cellular homeostasis.

Impairments in Sly1 expression or function relate to neurodegenerative diseases such as Alzheimer's disease. These conditions associate with defects in vesicular trafficking pathways as evidenced by altered Sly1 interactions with proteins like Syntaxin 5. Such interruptions can cause misprocessing and accumulation of proteins within neurons. Another disorder linked to Sly1 is developmental brain disorder where disruptions in vesicular fusion contribute to improper neuronal connections.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).
See full target information SCFD1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar20 PubMed38134218

2023

Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins.

Applications

Unspecified application

Species

Unspecified reactive species

Danièle Stalder,Igor Yakunin,Conceição Pereira,Jessica Eden,David C Gershlick

Human molecular genetics 21:586-608 PubMed22038835

2011

Loss of ceramide synthase 3 causes lethal skin barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Jennemann,Mariona Rabionet,Karin Gorgas,Sharon Epstein,Alexander Dalpke,Ulrike Rothermel,Aline Bayerle,Franciscus van der Hoeven,Silke Imgrund,Joachim Kirsch,Walter Nickel,Klaus Willecke,Howard Riezman,Hermann-Josef Gröne,Roger Sandhoff
View all publications
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