Anti-SLUG抗体(ab27568)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
概述
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产品名称
Anti-SLUG抗体
参阅全部 SLUG 一抗 -
描述
兔多克隆抗体to SLUG -
宿主
Rabbit -
特异性
ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates. -
经测试应用
适用于: WB, IHC-Frmore details
不适用于: ICC -
种属反应性
与反应: Mouse, Rat, Chicken, Human
预测可用于: Cow, Dog, Pig, Xenopus laevis -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27568于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa). |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
组织特异性
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
疾病相关
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
序列相似性
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 432368 Chicken
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 25554 Rat
- Entrez Gene: 379440 Xenopus laevis
- Omim: 602150 Human
- SwissProt: Q3MHQ4 Cow
- SwissProt: O43623 Human
see all -
别名
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (ab27568)This image is courtesy of Don Newgreen and Dong Cheng Zhang, Murdoch Childrens Research InstituteTransverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
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All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaSecondary antibody - goat anti-rabbit HRP (preadsorbed)
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All lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 lysate overexpressing SNAIL protein
Lane 3 : MCF7 lysate tranfected with vector control plasmid
Secondary
All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (162)
ab27568 被引用在 162 文献中.
- Kuang Y et al. KIAA1429 mediates epithelial mesenchymal transition in sorafenib-resistant hepatocellular carcinoma through m6A methylation modification. Cancer Med 12:7222-7233 (2023). PubMed: 36420693
- Adhikary G et al. Mesothelioma cancer cells are glutamine addicted and glutamine restriction reduces YAP1 signaling to attenuate tumor formation. Mol Carcinog 62:438-449 (2023). PubMed: 36562471
- Cheng Y et al. KCNK9 mediates the inhibitory effects of genistein on hepatic metastasis from colon cancer. Clinics (Sao Paulo) 78:100141 (2023). PubMed: 36905879
- Gong X et al. USP14 predicts poorer survival outcomes and promotes tumor progression in endometrial carcinoma by activating NF-κB signaling. Aging (Albany NY) 15:12120-12135 (2023). PubMed: 37917013
- He H et al. Downregulation of TAB182 promotes cancer stem-like cell properties and therapeutic resistance in triple-negative breast cancer cells. BMC Cancer 23:1101 (2023). PubMed: 37953246