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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-SLUG抗体(ab27568)

  • Datasheet
  • SDS
Reviews (11)Q&A (3)References (162)

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Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (ab27568)
  • Western blot - Anti-SLUG antibody (ab27568)
  • Western blot - Anti-SLUG antibody (ab27568)

Key features and details

  • Rabbit polyclonal to SLUG
  • Suitable for: WB, IHC-Fr
  • Reacts with: Mouse, Rat, Chicken, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SLUG抗体
    参阅全部 SLUG 一抗
  • 描述

    兔多克隆抗体to SLUG
  • 宿主

    Rabbit
  • 特异性

    ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates.
  • 经测试应用

    适用于: WB, IHC-Frmore details
    不适用于: ICC
  • 种属反应性

    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Stem Cells
    • Neural Stem Cells
    • Neural Crest Stem Cells
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Nervous system development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SLUG protein (ab201878)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab27568于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).
IHC-Fr (2)
Use at an assay dependent concentration.
说明
WB
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).
IHC-Fr
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC.

靶标

  • 功能

    Transcriptional repressor. Involved in the generation and migration of neural crest cells.
  • 组织特异性

    Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 疾病相关

    Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
  • 序列相似性

    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession O43623 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 432368 Chicken
    • Entrez Gene: 6591 Human
    • Entrez Gene: 20583 Mouse
    • Entrez Gene: 25554 Rat
    • Entrez Gene: 379440 Xenopus laevis
    • Omim: 602150 Human
    • SwissProt: Q3MHQ4 Cow
    • SwissProt: O43623 Human
    • SwissProt: P97469 Mouse
    • SwissProt: O08954 Rat
    • SwissProt: Q91924 Xenopus laevis
    • Unigene: 360174 Human
    • Unigene: 4272 Mouse
    • Unigene: 43117 Rat
    • Unigene: 3818 Xenopus laevis
    see all
  • 别名

    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein snail homolog 2 antibody
    • Slug (chicken homolog) zinc finger protein antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SNAI 2 antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI2 antibody
    see all

图片

  • Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (ab27568)
    Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (ab27568)This image is courtesy of Don Newgreen and Dong Cheng Zhang, Murdoch Childrens Research Institute
    Transverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
  • Western blot - Anti-SLUG antibody (ab27568)
    Western blot - Anti-SLUG antibody (ab27568)
    All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    Secondary antibody - goat anti-rabbit HRP (preadsorbed)

  • Western blot - Anti-SLUG antibody (ab27568)
    Western blot - Anti-SLUG antibody (ab27568)This image is courtesy of Archana Dhasarathy, Ph.D., NC
    All lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate overexpressing SLUG protein
    Lane 2 : MCF7 lysate overexpressing SNAIL protein
    Lane 3 : MCF7 lysate tranfected with vector control plasmid

    Secondary
    All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?



    ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (162)

发表研究结果有使用 ab27568?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab27568 被引用在 162 文献中.

  • Kuang Y  et al. KIAA1429 mediates epithelial mesenchymal transition in sorafenib-resistant hepatocellular carcinoma through m6A methylation modification. Cancer Med 12:7222-7233 (2023). PubMed: 36420693
  • Adhikary G  et al. Mesothelioma cancer cells are glutamine addicted and glutamine restriction reduces YAP1 signaling to attenuate tumor formation. Mol Carcinog 62:438-449 (2023). PubMed: 36562471
  • Cheng Y  et al. KCNK9 mediates the inhibitory effects of genistein on hepatic metastasis from colon cancer. Clinics (Sao Paulo) 78:100141 (2023). PubMed: 36905879
  • Gong X  et al. USP14 predicts poorer survival outcomes and promotes tumor progression in endometrial carcinoma by activating NF-κB signaling. Aging (Albany NY) 15:12120-12135 (2023). PubMed: 37917013
  • He H  et al. Downregulation of TAB182 promotes cancer stem-like cell properties and therapeutic resistance in triple-negative breast cancer cells. BMC Cancer 23:1101 (2023). PubMed: 37953246
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 14 Abreviews or Q&A

IHC - Wholemount abreview for Anti-SLUG antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Zebrafish Embryo (wholemount)
Specification
wholemount
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 04 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-SLUG antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Human Cell (mammalian cancer cells)
Specification
mammalian cancer cells
Permeabilization
Yes - Tween20
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. XXXXXX XXXXXX

Verified customer

提交于 Jul 01 2013

Immunohistochemistry (Frozen sections) abreview for Anti-SLUG antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E10.5 mouse embryo sections)
Specification
E10.5 mouse embryo sections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS / 0.5% v/v TritonX100 (Sigma)
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 11 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SLUG antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung adenocarcinoma induced by urethane)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Lung adenocarcinoma induced by urethane
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 08 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SLUG antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart, pancreas)
Specification
heart, pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
casein based (Biocare) as blocking agent for 10 minute(s) · Concentration: 00% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Julia Abramowitz

Verified customer

提交于 Nov 04 2009

Western blot abreview for Anti-SLUG antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (skin, comma D ß cell line)
Loading amount
40 µg
Specification
skin, comma D ß cell line
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Mayssaa NASSOUR

Verified customer

提交于 Jul 10 2009

Immunohistochemistry (Frozen sections) abreview for Anti-SLUG antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Cell (Embryonic section of spinal cord, stage 10-11)
Specification
Embryonic section of spinal cord, stage 10-11
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. James Briscoe

Verified customer

提交于 Nov 10 2008

Western blot abreview for Anti-SLUG antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (smooth muscle cells)
Loading amount
20 µg
Specification
smooth muscle cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 24 2008

Question

I can't get it work with IHC at all.


sample - rat embryos, paraffin embedded; 4% PFA fix for 24 hrs
AR - 10mM sodium citrate buffer
perm - 4% PBS-triton 100 1 hr
Block - 3% donkey serum + 1%BSA 1 hr at RT
1' ab - 1:250 - 1:1000 o/n at 4oC
2' ab - dk x rb biotin 1:200 1 hr at RT
ABC kit, DAB detection
positive control is nerve tissue

Read More

Abcam community

Verified customer

Asked on Aug 09 2012

Answer

Thank you for contacting Abcam regarding ab27568.


I am sorry to hear that a customer is experiencing difficulties with this antibody in IHC. Thank you for passing along the protocol information. Overall this looks pretty standard, and based on how the antibody was tested, should be compatible.


One point of interest is the sample being used by the customer. She indicates she used a rat embryo.Were whole embryos fixed and embedded and then sectioned? What tissue type? How thick were the sections? I am assuming she is not getting any staining at all? Even in her positive control?


I look forward to the customer's reply so that I may assist with further troubleshooting or a replacement. Please do not hesitate to let me know if she has any further questions!

Read More

Abcam Scientific Support

回复于 Aug 09 2012

Question


Hemos recibido la información sobre la alternativa al anti-SLUG que no se encuentra disponible. No estamos seguros de querer adquirir el anticuerpo alternativo, porque no ha sido utilizado en otros organismos. Puede decirnos por favor cuál es la secuencia que reconoce este anticuerpo, y si se trata de una secuencia conservada?

Read More

Abcam community

Verified customer

Asked on Mar 16 2012

Answer

Gracias por contactarnos. Te pido disculpas por la espera, he contactado con mis compañeros del laboratorio para poder darte una información más completa.

La secuencia de aminoácidos del inmunógeno de ab51772 es XXXXXXXXXX . Esta secuencia coincide con la secuencia del inmunógeno del anticuerpo ab27568 que no se encuentra actualmente en stock, por lo que si este anticuerpo os funcionó en el pasado, la alternativa monoclonal debería daros resultados similares.

La secuencia de la proteína es razonablemente conservada en mamíferos, pero no tenemos suficientes referencias para afirmar lo mismo en Oryzias latipes. He intentado llevar a cabo un alineamiento de secuencias pero no he encontrado ni la secuencia de DNA ni la proteica para O. Latines. Es posible que no se encuentre todavía en Genbank (o quizás lo esté con un nombre diferente).

Espero que esta información te sea útil, de lo contrario, por favor no dudes en volver a contactarnos.

Read More

Abcam Scientific Support

回复于 Mar 16 2012

1-10 of 14 Abreviews or Q&A

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