Anti-SLPI抗体- Aminoterminal end (ab46763)
Key features and details
- Rabbit polyclonal to SLPI - Aminoterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SLPI抗体- Aminoterminal end
参阅全部 SLPI 一抗 -
描述
兔多克隆抗体to SLPI - Aminoterminal end -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide based on the aminoterminal end of human SLPI.(Please note: the amino acid sequence is proprietary) (Peptide available as ab46833.)
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阳性对照
- WB: Recombinant Human SLP I, Wild-type HeLa cell lysate, PC-3 cell lysate, LNCaP cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46763于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 14 kDa.
A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 14 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
靶标
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功能
Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. -
组织特异性
Mucous fluids. -
序列相似性
Contains 2 WAP domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6590 Human
- GenBank: NM_003064 Human
- Omim: 107285 Human
- SwissProt: P03973 Human
- Unigene: 517070 Human
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别名
- ALK 1 antibody
- ALK1 antibody
- ALP antibody
see all
图片
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Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SLPI knockout HeLa cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line ab265215 (knockout cell lysate ab257685). To generate this image, wild-type and SLPI knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye®800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1 : Recombinant Human SLP I at 0.04 µg
Lane 2 : Recombinant Human SLP I at 0.02 µg
Lane 3 : Recombinant Human SLP I at 0.01 µg
Lane 4 : Recombinant Human SLP I at 0.004 µg
Lane 5 : Recombinant Human SLP I at 0.001 µg
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab46763 被引用在 2 文献中.
- Klimenkova O et al. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood 123:1239-49 (2014). Human . PubMed: 24352879
- Yang Y et al. Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker. PLoS One 9:e95389 (2014). WB ; Human . PubMed: 24748380