重组Anti-SLP76 (phospho Y113)抗体[EPR2851] (ab75990)
![Western blot - Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)](https://www.abcam.cn/ps/products/75/ab75990/Images/ab75990-91240-anti-slp76-phospho-y113-antibody-epr2851-western-blot.jpg "Western blot - Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SLP76 (phospho Y113)抗体[EPR2851]
参阅全部 SLP76 一抗 -
描述
兔单克隆抗体[EPR2851] to SLP76 (phospho Y113) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Rabbit
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat cytoplasmic lysate treated with pervanadate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2851 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75990于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
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| 说明 |
|---|
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WB
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa). |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Involved in T-cell antigen receptor mediated signaling. -
组织特异性
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. -
序列相似性
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain. -
结构域
The SH2 domain mediates interaction with SHB. -
翻译后修饰
Phosphorylated after T-cell receptor activation by ZAP-70. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3937 Human
- Omim: 601603 Human
- SwissProt: Q13094 Human
- Unigene: 304475 Human
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别名
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
see all
图片
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Western blot - Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)All lanes : Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990) at 1/10000 dilution
Lane 1 : Jurkat cell lysate - untreated
Lane 2 : Jurkat cell lysate - treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted? -
![Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)](https://www.abcam.cn/ps/products/75/ab75990/Images/ab75990-2-benefits-of-recombinant-antibodies.png)
Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab75990 被引用在 3 文献中.
- Hoshino A et al. Dysregulation of Epstein-Barr Virus Infection in Hypomorphic ZAP70 Mutation. J Infect Dis 218:825-834 (2018). PubMed: 29684201
- Cimo AM et al. CD25 and CD69 induction by a4ß1 outside-in signalling requires TCR early signalling complex proteins. Biochem J 454:109-21 (2013). WB ; Human . PubMed: 23758320
- Köhler K et al. Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells. PLoS One 5:e15374 (2010). WB ; Human . PubMed: 21179506