Anti-SLP76抗体(ab17029)
Key features and details
- Goat polyclonal to SLP76
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SLP76抗体
参阅全部 SLP76 一抗 -
描述
山羊多克隆抗体to SLP76 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
ALRNVPFRSEV
, corresponding to amino acids 2-12 of Human SLP76. -
阳性对照
- Recombinant Human SLP76 protein (ab114697) can be used as a positive control in WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17029于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Involved in T-cell antigen receptor mediated signaling. -
组织特异性
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. -
序列相似性
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain. -
结构域
The SH2 domain mediates interaction with SHB. -
翻译后修饰
Phosphorylated after T-cell receptor activation by ZAP-70. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3937 Human
- Omim: 601603 Human
- SwissProt: Q13094 Human
- Unigene: 304475 Human
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别名
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
see all
图片
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Western blot - Anti-SLP76 antibody (ab17029)Anti-SLP76 antibody (ab17029) at 0.3 µg/ml + Jurkat lysate (protein in RIPA buffer) at 35 µg
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (1)
ab17029 被引用在 1 文献中.
- Lev A et al. Inherited SLP76 deficiency in humans causes severe combined immunodeficiency, neutrophil and platelet defects. J Exp Med 218:N/A (2021). PubMed: 33231617
