Anti-Slit3抗体(ab11018)
Key features and details
- Rabbit polyclonal to Slit3
- Suitable for: WB
- Reacts with: Cow
- Isotype: IgG
概述
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产品名称
Anti-Slit3抗体
参阅全部 Slit3 一抗 -
描述
兔多克隆抗体to Slit3 -
宿主
Rabbit -
特异性
A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Cow
预测可用于: Mouse, Rat, Chicken, Human
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免疫原
Synthetic peptide:
GKDSYVELASAKVR-C
conjugated to KLH, corresponding to amino acids 1164-1177 of Human Slit3. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11018于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 145 kDa (predicted molecular weight: 169 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 145 kDa (predicted molecular weight: 169 kDa). |
靶标
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功能
May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors. -
组织特异性
Predominantly expressed in thyroid. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 374173 Chicken
- Entrez Gene: 6586 Human
- Entrez Gene: 20564 Mouse
- Entrez Gene: 83467 Rat
- Omim: 603745 Human
- SwissProt: O75094 Human
- SwissProt: Q9WVB4 Mouse
- SwissProt: O88280 Rat
see all -
别名
- FLJ10764 antibody
- KIAA0814 antibody
- MEGF 5 antibody
see all
图片
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Western blot - Anti-Slit3 antibody (ab11018)Anti-Slit3 antibody (ab11018) at 1/800 dilution + Bovine thyroid whole cell lysate at 35 µg
Secondary
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 169 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab11018 被引用在 2 文献中.
- Kong Y et al. Slit-miR-218-Robo axis regulates retinal neovascularization. Int J Mol Med 37:1139-45 (2016). PubMed: 26935869
- Pi L et al. CCN2/CTGF regulates neovessel formation via targeting structurally conserved cystine knot motifs in multiple angiogenic regulators. FASEB J : (2012). WB . PubMed: 22611085