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AB11018

Anti-Slit3抗体

Anti-Slit3 antibody

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(2 Publications)

Rabbit Polyclonal SLIT3 antibody. Suitable for WB and reacts with Cow samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLIT3 aa 1150-1200 conjugated to Keyhole Limpet Haemocyanin.

查看别名

KIAA0814, MEGF5, SLIL2, UNQ691/PRO1336, SLIT3, Slit homolog 3 protein, Slit-3, Multiple epidermal growth factor-like domains protein 5, Multiple EGF-like domains protein 5

1 Images
Western blot - Anti-Slit3 antibody (AB11018)
  • WB

Unknown

Western blot - Anti-Slit3 antibody (AB11018)

All lanes:

Western blot - Anti-Slit3 antibody (ab11018) at 1/800 dilution

All lanes:

Bovine thyroid whole cell lysate at 35 µg

Secondary

All lanes:

Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 168 kDa

Observed band size: 145 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Cow

应用

WB

applications

免疫原

Synthetic Peptide within Human SLIT3 aa 1150-1200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O75094

特异性

This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Slit3 also known as Slit Homolog 3 is a secreted protein encoded by the SLIT3 gene. It belongs to the Slit family of proteins and has a mass of approximately 160 kDa. These proteins typically have a leucine-rich domain and immunoglobulin-like repeats. Slit3 is expressed in various tissues including the heart lung and kidney but shows highest expression in the fetal lung. It operates in the extracellular space where it interacts with cell surface receptors to relay information essential for cellular functions.
Biological function summary

Slit3 regulates cellular migration and axon guidance by binding to Roundabout (Robo) receptors. This interaction plays a critical role in neuronal development and vascularization processes. In addition Slit3 can influence endothelial cell behavior and is a part of the axon guidance complex. By contributing to angiogenesis and neural patterning Slit3 impacts both developmental and repair mechanisms within the organism.

Pathways

The Slit3 protein participates in the axon guidance and angiogenesis signaling pathways. Slit3 interacts with Robo receptors to mediate cellular repulsion and navigation of the growth cones influencing nervous system architecture. The protein is closely related to other Slit family members particularly Slit1 and Slit2 which share similar roles in regulating guidance cues and vascular formation ensuring proper tissue development and injury response.

Disruptive mutations or altered expression of Slit3 have been linked to congenital diaphragmatic hernias and tumorigenesis. Congenital diaphragmatic hernia a defect that affects the diaphragm development connects to abnormal Slit3-mediated signaling that impacts tissue differentiation. Additionally changes in Slit3 expression might contribute to cancer progression by affecting tumor angiogenesis; this interaction involves proteins such as Robo1 and Robo4 making them potential targets in disease modulation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
See full target information SLIT3
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 37:1139-45 PubMed26935869

2016

Slit-miR-218-Robo axis regulates retinal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Yichun Kong,Bei Sun,Quanhong Han,Shuang Han,Yuchuan Wang,Ying Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26:3365-79 PubMed22611085

2012

CCN2/CTGF regulates neovessel formation via targeting structurally conserved cystine knot motifs in multiple angiogenic regulators.

Applications

WB

Species

Unspecified reactive species

Liya Pi,Anitha K Shenoy,Jianwen Liu,Seungbum Kim,Nikole Nelson,Huiming Xia,William W Hauswirth,Bryon E Petersen,Gregory S Schultz,Edward W Scott
View all publications
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