Anti-Slit2抗体(ab7665)
Key features and details
- Rabbit polyclonal to Slit2
- Suitable for: WB, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Slit2抗体
参阅全部 Slit2 一抗 -
描述
兔多克隆抗体to Slit2 -
宿主
Rabbit -
特异性
A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.
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经测试应用
适用于: WB, ICC/IF, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human
预测可用于: Xenopus laevis
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免疫原
Synthetic peptide:
YFIPGTEDYRSKLSGDC
conjugated to KLH, corresponding to amino acids 484-500 of Human SLIT2. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This affinity purified antibody is directed against human SLIT-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal.
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 20498081
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| 说明 |
|---|
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WB
1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 20498081 |
靶标
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功能
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. -
组织特异性
Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
结构域
The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro. -
细胞定位
Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. - Information by UniProt
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数据库链接
- Entrez Gene: 373967 Chicken
- Entrez Gene: 9353 Human
- Entrez Gene: 20563 Mouse
- Entrez Gene: 360272 Rat
- Entrez Gene: 394404 Xenopus laevis
- Omim: 603746 Human
- SwissProt: O94813 Human
- SwissProt: Q9R1B9 Mouse
see all -
别名
- Drad 1 antibody
- E030015M03Rik antibody
- E130320P19Rik antibody
see all
图片
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Western blot - Anti-Slit2 antibody (ab7665)Anti-Slit2 antibody (ab7665) at 1/1350 dilution + Chicken spinal cord whole cell lysate at 30 µg
Secondary
IRDye™800
conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 170 kDa
Observed band size: 165 kDa why is the actual band size different from the predicted?Molecular weight estimation was made by comparison to prestained molecular weight markers
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab7665 被引用在 13 文献中.
- Hamberger F et al. An inducible model for genetic manipulation and fate-tracing of PDGFRβ-expressing fibrogenic cells in the liver. Sci Rep 13:7322 (2023). PubMed: 37147343
- Li X et al. Slit2 Protects Hearts Against Ischemia-Reperfusion Injury by Inhibiting Inflammatory Responses and Maintaining Myofilament Contractile Properties. Front Physiol 11:228 (2020). PubMed: 32292352
- Zheng Y et al. The influence of retinoic acid-induced differentiation on the radiation response of male germline stem cells. DNA Repair (Amst) 70:55-66 (2018). PubMed: 30179733
- Sakima M et al. VEGFR-3 signaling is regulated by a G-protein activator, activator of G-protein signaling 8, in lymphatic endothelial cells. Exp Cell Res 368:13-23 (2018). Human . PubMed: 29649427
- Piao JM et al. MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage. Cell Physiol Biochem 46:890-906 (2018). PubMed: 29669322