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AB10984

Anti-Slit1抗体

Anti-Slit1 antibody

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Rabbit Polyclonal SLIT1 antibody. Suitable for WB and reacts with Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Mouse Slit1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.

查看别名

Kiaa0813, Slit1, Slit homolog 1 protein, Slit-1

1 Images
Western blot - Anti-Slit1 antibody (AB10984)
  • WB

Unknown

Western blot - Anti-Slit1 antibody (AB10984)

ab10984-Rabbit polyclonal to Slit1 (1/500)

Secondary ab : Goat anti-rabbit IgG ab6721 (1/5000); 20μg of cell lysate per lane. Expected molecular weight : 168 kDa; Exposure time : 5 min

Lane 1 : Rat Brain tissue lysate
Lane 2 : Mouse Brain tissue Lysate

Note : The smaller strong bands observed in this WB are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).

ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab : Goat anti-rabbit HRP ab6721 (1/5000); 20μg of cell lysate per lane. Expected molecular weight : 168 kDa; Exposure time : 5 min Lane 1 : Rat Brain tissue lysate Lane 2 : Mouse Brain tissue Lysate Note : The smaller strong bands observed in this WB are likely to be cleavage products. A number of

All lanes:

Western blot - Anti-Slit1 antibody (ab10984)

Predicted band size: 168 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat

应用

WB

applications

免疫原

Synthetic Peptide within Mouse Slit1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q80TR4

特异性

The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).

This affinity purified antibody is directed against mouse SLIT-1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
This affinity purified antibody is directed against mouse SLIT-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Slit1 also known as Slit homolog 1 protein is a large extracellular matrix protein with a mass of approximately 200 kDa. This protein is expressed in the developing and adult nervous system particularly in the brain. It contributes to axon guidance and cellular migration through its interaction with Robo receptors. By serving as a secreted ligand Slit1 plays a critical role in nervous system development guiding neurons to their correct positions.
Biological function summary

Slit1 facilitates the formation of neural circuits by promoting axonal repulsion during neural development. It interacts as part of a receptor-ligand complex with Robo receptors on the cell surface which triggers intracellular signaling cascades. Slit1 also influences the shaping of synaptic connections and acts in a chemorepulsive manner ensuring appropriate neural wiring. Its presence aids cellular organization impacting cell division and movement in the nervous system.

Pathways

Slit1 protein is an integral player in the axon guidance and neuronal network formation pathways. In these processes it modulates actin cytoskeleton dynamics and affects growth cone collapse through signaling with proteins like Robo1 and Robo2. The Slit-Robo signaling pathway works to coordinate cell movement and communication within the developing brain aligning with pathways that involve other guidance cues like Netrins and Semaphorins for balanced nervous system organization.

Slit1 has connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysfunction can disrupt normal neural pathways contributing to improper brain connectivity and circuit formation. Slit1 interacts with other proteins implicated in these disorders such as Netrin-1 which supports its role in maintaining neural network integrity. Understanding Slit1's involvement provides insights into potential therapeutic targets for treating such developmental and neurological disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
See full target information Slit1
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