Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-SLIRP抗体[1E10BC8] (ab119687)

Submit a review Submit a question
Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)
  • Flow Cytometry - Anti-SLIRP antibody [1E10BC8] (ab119687)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLIRP antibody [1E10BC8] (ab119687)

Key features and details

  • Mouse monoclonal [1E10BC8] to SLIRP
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-SLIRP抗体[1E10BC8]
    参阅全部 SLIRP 一抗

  • 描述

    小鼠单克隆抗体[1E10BC8] to SLIRP

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • 阳性对照

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab119687于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.

靶标

  • 相关性

    SLIRP is a RNA binding protein that acts as a nuclear receptor corepressor. It may act by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. SLIRP binds the STR7 loop of SRA RNA. It is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It contains 1 RRM (RNA recognition motif) domain. SLIRP is predominantly founs in mitochondrial with a limited role in the nucleus where it is recruited to nuclear receptor target promoters.

  • 细胞定位

    Mitochondrial and Nuclear; Predominantly mitochondrial.

  • 数据库链接

    • 别名

      • C14orf156 antibody
      • Chromosome 14 open reading frame 156 antibody
      • DC50 antibody
      • PD04872 antibody
      • SLIRP antibody
      • SRA stem loop interacting RNA binding protein, mitochondrial antibody
      see all

    图片

    • Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)
      All lanes : Anti-SLIRP antibody [1E10BC8] (ab119687) at 1 µg/ml

      Lane 1 : HepG2 lysate at 20 µg
      Lane 2 : fibroblast lysate at 20 µg/ml
      Lane 3 : SLIRP recombinant protein at 0.01 µg

      Secondary
      All lanes : Infra red fluorescence - Goat anti-mouse 800 at 1/5000 dilution

      Predicted band size: 12 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Immunocytochemistry of ab119687 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab119687, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
    • Flow Cytometry - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Flow Cytometry - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Fibroblast (HDFn) cells were stained with 1µg/mL SLIRP antibody (ab119687) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLIRP antibody [1E10BC8] (ab119687)

      IHC image of SLIRP staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119687, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    文献 (0)

    发表研究结果有使用 ab119687？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119687 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab119687.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com