Anti-SLFN5抗体(ab121537)
Key features and details
- Rabbit polyclonal to SLFN5
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SLFN5抗体 -
描述
兔多克隆抗体to SLFN5 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to internal sequence amino acids 70-210 of Human SLFN5.
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阳性对照
- ICC/IF: A-431 human cell line IHC: Human lymph node, Fallopian tube and endometrium tissue Wb: SK-MEL-30 and Caco-2 cell lines
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
May have a role in hematopoeitic cell differentiation. -
序列相似性
Belongs to the Schlafen family. - Information by UniProt
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数据库链接
- Entrez Gene: 162394 Human
- SwissProt: Q08AF3 Human
- Unigene: 709347 Human
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别名
- AI956840 antibody
- RP23-392I13.5 antibody
- Schlafen 5 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)
ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human fallopian tube by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human endometrium by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human lymph node by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-SLFN5 antibody (ab121537)Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-SLFN5 antibody (ab121537)All lanes : Anti-SLFN5 antibody (ab121537) at 0.4 µg/ml
Lane 1 : SK-MEL-30 cell lysate
Lane 2 : Caco-2 cell lysateLoading control: Anti-HDAC1
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab121537 被引用在 5 文献中.
- Ding J et al. Schlafen-5 inhibits LINE-1 retrotransposition. iScience 26:107968 (2023). PubMed: 37810251
- Ding J et al. Schlafen 5 suppresses human immunodeficiency virus type 1 transcription by commandeering cellular epigenetic machinery. Nucleic Acids Res 50:6137-6153 (2022). PubMed: 35687115
- Martinez RS et al. SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer. Cancer Res 81:3664-3678 (2021). PubMed: 33985973
- Companioni Nápoles O et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). PubMed: 27032393
- Ding L et al. Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia. J Clin Invest 126:2867-80 (2016). PubMed: 27427984