Anti-SLFN11抗体(ab121731)
Key features and details
- Rabbit polyclonal to SLFN11
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SLFN11抗体
参阅全部 SLFN11 一抗 -
描述
兔多克隆抗体to SLFN11 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: REVLGCAKEN VDPDSLRRKI EQAIYKLPCV HFCQPQRPIT FTLKIVDVLK RGELYGYACM IRVNPFCCAV FSEAPNSWI, corresponding to amino acids 255-333 of Human SLFN11.
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阳性对照
- Human pancreas tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 103 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 103 kDa. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the Schlafen family. - Information by UniProt
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数据库链接
- Entrez Gene: 91607 Human
- SwissProt: Q7Z7L1 Human
- Unigene: 462829 Human
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别名
- Schlafen 11 antibody
- Schlafen antibody
- Schlafen family member 11 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SLFN11 antibody (ab121731)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121731 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-SLFN11 antibody (ab121731)Anti-SLFN11 antibody (ab121731) + MOLT-4
Secondary
SLFN11
Predicted band size: 103 kDa
Observed band size: 103 kDaWestern blot analysis in human cell line MOLT-4 staining SLFN11 with ab121731
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN11 antibody (ab121731)ab121731 at 1/15 dilution staining SLFN11 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in islet cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab121731 被引用在 5 文献中.
- Bednarikova M et al. Can Schlafen 11 Help to Stratify Ovarian Cancer Patients Treated with DNA-Damaging Agents? Cancers (Basel) 14:N/A (2022). PubMed: 35625957
- Maifrede S et al. TET2 and DNMT3A Mutations Exert Divergent Effects on DNA Repair and Sensitivity of Leukemia Cells to PARP Inhibitors. Cancer Res 81:5089-5101 (2021). PubMed: 34215619
- Yap TA et al. Ceralasertib (AZD6738), an Oral ATR Kinase Inhibitor, in Combination with Carboplatin in Patients with Advanced Solid Tumors: A Phase I Study. Clin Cancer Res 27:5213-5224 (2021). PubMed: 34301752
- Winkler C et al. SLFN11 captures cancer-immunity interactions associated with platinum sensitivity in high-grade serous ovarian cancer. JCI Insight 6:N/A (2021). PubMed: 34549724
- Willis SE et al. Retrospective analysis of Schlafen11 (SLFN11) to predict the outcomes to therapies affecting the DNA damage response. Br J Cancer 125:1666-1676 (2021). PubMed: 34663950