Anti-SLCO1B3/OATP1B3抗体(ab224064)
Key features and details
- Rabbit polyclonal to SLCO1B3/OATP1B3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SLCO1B3/OATP1B3抗体
参阅全部 SLCO1B3/OATP1B3 一抗 -
描述
兔多克隆抗体to SLCO1B3/OATP1B3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SLCO1B3/OATP1B3 aa 650 to the C-terminus.
Database link: Q9NPD5 -
阳性对照
- IHC-P: Human liver tissue; ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224064于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Mediates the Na(+)-independent uptake of organic anions such as 17-beta-glucuronosyl estradiol, taurocholate, triiodothyronine (T3), leukotriene C4, dehydroepiandrosterone sulfate (DHEAS), methotrexate and sulfobromophthalein (BSP). Involved in the clearance of bile acids and organic anions from the liver. -
组织特异性
Highly expressed in liver, in particular at the basolateral membrane of hepatocytes near the central vein. Not detected in other tissues. Highly expressed in some cancer cell lines derived from colon, pancreas, liver and gall bladder. -
疾病相关
Hyperbilirubinemia, Rotor type -
序列相似性
Belongs to the organo anion transporter (TC 2.A.60) family.
Contains 1 Kazal-like domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Basolateral cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 28234 Human
- Omim: 605495 Human
- SwissProt: Q9NPD5 Human
- Unigene: 504966 Human
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别名
- HBLRR antibody
- Liver-specific organic anion transporter 2 antibody
- LST-2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Immunohistochemical analysis of human liver tissue labeling SLCO1B3/OATP1B3 in the membrane of hepatocytes with ab224064 at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (ab224064)Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling SLCO1B3/OATP1B3 (green) in the plasma membrane with ab224064 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)Immunohistochemical analysis of human cerebral cortex tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)Immunohistochemical analysis of human skin tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)Immunohistochemical analysis of human kidney tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)Paraffin embedded human kidney tissue stained for SLCO1B3/OATP1B3 with ab224064 (1/1000 dilution) in immunohistochemical analysis. Negative control - no positive staining in kidney tissue.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab224064 被引用在 2 文献中.
- Ronaldson PT et al. Transport Properties of Statins by Organic Anion Transporting Polypeptide 1A2 and Regulation by Transforming Growth Factor-β Signaling in Human Endothelial Cells. J Pharmacol Exp Ther 376:148-160 (2021). PubMed: 33168642
- Sapp V et al. Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity. Sci Rep 11:13866 (2021). PubMed: 34230586