Anti-SLC7A6OS抗体(ab122727)
Key features and details
- Rabbit polyclonal to SLC7A6OS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SLC7A6OS抗体 -
描述
兔多克隆抗体to SLC7A6OS -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: AEALVLACKR LRSDAVESAA QKTSEGLERA AENNVFHLVA TVCSQEEPVQ PLLREVLRPS RDSQQRVRRN LRASAREVRQ EGRYRVLS, corresponding to amino acids 20-107 of Human SLC7A6OS.
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阳性对照
- WB: Transfected HEK293T whole cells lysates; IHC-P: Human testis tissue; ICC: U-2 OS whole cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122727于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 说明 |
|---|
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
靶标
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序列相似性
Belongs to the SLC7A6OS family. - Information by UniProt
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数据库链接
- Entrez Gene: 84138 Human
- SwissProt: Q96CW6 Human
- Unigene: 709557 Human
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别名
- 2010007L18Rik antibody
- 2400002F02Rik antibody
- ADAMS proteinase-related protein antibody
see all
图片
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Western blot - Anti-SLC7A6OS antibody (ab122727)All lanes : Anti-SLC7A6OS antibody (ab122727) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SLC7A6OS over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC7A6OS antibody (ab122727)ab122727, at 1/500 dilution, staining SLC7A6OS in paraffin-embedded Human testis tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-SLC7A6OS antibody (ab122727)Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labelling SLC7A6OS with ab122727 shows positivity in the nucleus but is excluded from the nucleoli.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122727 被引用在 1 文献中.
- Mazzola L et al. Progressive Myoclonus Epilepsy Caused by a Homozygous Splicing Variant of SLC7A6OS. Ann Neurol 89:402-407 (2021). PubMed: 33085104