Anti-SLC51B抗体(ab121285)
Key features and details
- Rabbit polyclonal to SLC51B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-SLC51B抗体 -
描述
兔多克隆抗体to SLC51B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SLC51B aa 77-127.
Sequence:VLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPET E
Database link: Q86UW2 -
阳性对照
- WB: Caco-2 cell lysate. IHC-P: Human small intestine, testis and kidney tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. -
组织特异性
Widely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat. -
序列相似性
Belongs to the OST-beta family. -
细胞定位
Cell membrane. Mainly restricted to the lateral and basal membranes of ileal enterocytes. - Information by UniProt
-
数据库链接
- Entrez Gene: 123264 Human
- Omim: 612085 Human
- SwissProt: Q86UW2 Human
- Unigene: 27018 Human
-
别名
- Organic solute transporter subunit beta antibody
- OST-beta antibody
- OSTB antibody
- OSTB_HUMAN antibody
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (ab121285)Immunohistochemical analysis of human testis tissue labeling OST beta with ab121285 at 1/50 dilution.
-
Western blot - Anti-SLC51B antibody (ab121285)All lanes : Anti-SLC51B antibody (ab121285) at 0.4 µg/ml
Lane 1 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
Observed band size: 14 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (ab121285)Immunohistochemical analysis of human kidney tissue labeling OST beta with ab121285 at 1/50 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (ab121285)Immunohistochemical analysis of human liver tissue labeling OST beta with ab121285 at 1/50 dilution. This showes no positivity as expected. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC51B antibody (ab121285)Immunohistochemical analysis of human small intestine tissue labeling OST beta with ab121285 at 1/50 dilution.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab121285 被引用在 3 文献中.
- Wang G et al. Development of a Bile Acid-Related Gene Signature for Predicting Survival in Patients with Hepatocellular Carcinoma. Comput Math Methods Med 2022:9076175 (2022). PubMed: 35592684
- Ramli MNB et al. Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease. Gastroenterology 159:1471-1486.e12 (2020). PubMed: 32553762
- Wang C et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). PubMed: 30043216