Anti-SLC4A4/NBC 抗体

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (AB187511)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification : 400x.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (AB187511)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification : 400x.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (AB187511)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification : 400x.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (AB187511)

Immunohistochemical analysis of paraffin embedded rat kidney tissue labeling SLC4A4/NBC with ab187511 at 1 : 100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (AB187511)

Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling SLC4A4/NBC with ab187511 at 1 : 100 dilution.

• WB

Supplier Data

Western blot - Anti-SLC4A4/NBC antibody (AB187511)

Blocking and diluting buffer and concentration : 3% NFDM/TBST.

All lanes:

Western blot - Anti-SLC4A4/NBC antibody (ab187511) at 1/1000 dilution

All lanes:

Lysates from LNCaP (Lymph Node Carcinoma of the Prostate) cells

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 121 kDa

Observed band size: 135 kDa

true

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-SLC4A4/NBC antibody (AB187511)

Blocking and diluting buffer and concentration : 3% NFDM/TBST.

All lanes:

Western blot - Anti-SLC4A4/NBC antibody (ab187511) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate

Lane 2:

Rat brain tissue lysate

Observed band size: 135 kDa

true

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-SLC4A4/NBC antibody (AB187511)

SLC4A4/NBC western blot using anti-SLC4A4/NBC antibody ab187511. Publication image and figure legend from Hwang, S., Shin, D. M., et al., 2019, Molecules, PubMed 31546841.

ab187511 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab187511 please see the product overview.

Surface protein expression of AE2 protein was decreased by DSF treatment. (A) Analysis of AE2 mRNA expression at rising concentrations from 0.1–4 μM DSF for 48 h (n = 5). (B) Protein expression of AE2 at indicated DSF concentration. (C) Analysis of relative AE2 intensity (n = 3, * p < 0.05, *** p < 0.001). (D) Immunostained images of AE2 (red), nucleus (DAPI, blue) and merge of cell morphology (TPM-T, gray) with and without DSF treatment. The scale bars represent 20 μm. (E) The surface expression of AE2 after treatment with 2 μM DSF. The β-actin and input AE2 blots were used as loading controls. (F) Analysis of relative intensity of AE2 for input and biotinylated (Bio) blot. Bars represent mean ± SEM (n = 3, ** p < 0.01). (G) Protein expressions of NBCe1 and NBCn1. (H) Analysis of relative intensity of NBCe1 and NBCn1 blots. Bars represent mean ± SEM. (I) Protein expressions of LC3B and p62. The β-actin blot was used as a loading control. (J) Analysis of relative intensity of LC3B and p62 blots. Bars represent mean ± SEM.

false