AB243938

重组Anti-SLC39A6/ZIP-6抗体[SP300] - BSA and Azide free

Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SLC39A6/ZIP-6 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

查看别名

LIV1, ZIP6, SLC39A6, Zinc transporter ZIP6, Estrogen-regulated protein LIV-1, Solute carrier family 39 member 6, Zrt- and Irt-like protein 6, ZIP-6

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227696).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227696).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] - BSA and Azide free (AB243938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling SLC39A6/ZIP-6 with ab227696 at 1/100 dilution (4.05 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227696)

不同偶联物与剂型 (1)

Unconjugated

Anti-SLC39A6/ZIP-6 antibody [SP300]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

SP300

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary antibody incubation for 10 minutes at room temperature." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab243938 is the carrier-free version of ab227696.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

纯化说明

Purified from TCS by protein A/G.

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SLC39A6 also known as ZIP-6 functions as a zinc transporter involved in importing zinc into cells from the extracellular environment. This protein is part of the ZIP family and has a mass of approximately 93 kDa. ZIP-6 is frequently expressed in tissues such as liver kidney and various epithelial cells. ZIP transporters like ZIP-6 play an important role in zinc homeostasis by facilitating cellular uptake of this essential trace element which impacts many cellular processes.

Biological function summary

ZIP-6 actively regulates intracellular zinc levels essential for many cellular functions like growth and development. It exists independently and does not directly form a part of a larger complex. ZIP-6 enhances zinc-dependent signaling pathways and is necessary for different cellular tasks like gene expression and enzyme activity. By controlling zinc entry into cells ZIP-6 supports several zinc-driven biological processes impacting cellular metabolism and survival.

Pathways

Researchers link ZIP-6 to the JAK-STAT signaling and MAPK pathways. In these pathways zinc acts as a necessary cofactor for numerous enzymes and transcription factors. ZIP-6's zinc transport activity connects with proteins such as STAT3 in the JAK-STAT pathway influencing cell proliferation and differentiation. ZIP-6's regulation of zinc concentrations affects the MAPK pathway which includes proteins like ERK1/2 highlighting its involvement in signal transduction and cellular response to stimuli.

ZIP-6 plays an essential role in cancer progression and immune system dysfunctions. Overexpression of ZIP-6 associates with breast cancer where it can promote proliferation and metastasis by affecting zinc-dependent pathways. ZIP-6 activity relates also to immune-related disorders influencing the inflammatory response through interactions with NF-kappaB. Changes in the activity or expression of ZIP-6 alter zinc homeostasis adding complexity to its disease-related impact.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Zinc-influx transporter which plays a role in zinc homeostasis and in the induction of epithelial-to-mesenchymal transition (EMT) (PubMed : 12839489, PubMed : 18272141, PubMed : 21422171, PubMed : 23919497, PubMed : 27274087, PubMed : 34394081). When associated with SLC39A10, the heterodimer formed by SLC39A10 and SLC39A6 mediates cellular zinc uptake to trigger cells to undergo epithelial- to-mesenchymal transition (EMT) (PubMed : 27274087). The SLC39A10-SLC39A6 heterodimer also controls NCAM1 phosphorylation and its integration into focal adhesion complexes during EMT (By similarity). Zinc influx inactivates GSK3B, enabling unphosphorylated SNAI1 in the nucleus to down-regulate adherence genes such as CDH1, causing loss of cell adherence (PubMed : 23919497). In addition, the SLC39A10-SLC39A6 heterodimer plays an essentiel role in initiating mitosis by importing zinc into cells to initiate a pathway resulting in the onset of mitosis (PubMed : 32797246). Participates in the T-cell receptor signaling regulation by mediating cellular zinc uptake into activated lymphocytes (PubMed : 21422171, PubMed : 30552163, PubMed : 34394081). Regulates the zinc influx necessary for proper meiotic progression to metaphase II (MII) that allows the oocyte-to-egg transition (PubMed : 25143461).

See full target information SLC39A6

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重组Anti-SLC39A6/ZIP-6抗体[SP300] - BSA and Azide free