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重组RabMAb

重组Anti-SLC38A5 / SNAT5抗体[EPR27109-86] - BSA and Azide free (ab317686)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
  • Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
  • Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
  • Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR27109-86] to SLC38A5 / SNAT5 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-SLC38A5 / SNAT5抗体[EPR27109-86] - BSA and Azide free
    参阅全部 SLC38A5 / SNAT5 一抗

  • 描述

    兔单克隆抗体[EPR27109-86] to SLC38A5 / SNAT5 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, Flow Cytmore details
    不适用于: ICC/IF,IP or WB

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human pancreas tissue, Human tonsil tissue, Hela cell pellet and A-549 cell pellet. Flow Cyt: HeLa and HepG2 cells.

  • 常规说明

    ab317686 is the carrier-free version of ab317685.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317686于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IP or WB.

靶标

  • 功能

    Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium.

  • 组织特异性

    Predominantly expressed in stomach, brain, liver, lung and intestinal tract.

  • 序列相似性

    Belongs to the amino acid/polyamine transporter 2 family.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Amino acid transporter system N2 antibody
      • JM 24 antibody
      • JM24 antibody
      • pp7194 antibody
      • S38A5 antibody
      • S38A5_HUMAN antibody
      • Slc38a5 antibody
      • SLC8 A5 antibody
      • SLC8A 5 antibody
      • SN 2 antibody
      • SN2 antibody
      • SNAT 5 antibody
      • SNAT5 antibody
      • Sodium coupled neutral amino acid transporter 5 antibody
      • Sodium coupled neutral amino acid transporter5 antibody
      • Sodium-coupled neutral amino acid transporter 5 antibody
      • Solute carrier family 38 member 5 antibody
      • Solute carrier family 38 member5 antibody
      • System N transporter 2 antibody
      • System N transporter2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

      This data was developed using ab317685, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human pancreas (PMID: 37887353).
      The section was incubated with ab317685 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

      This data was developed using ab317685, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on endothelium of human tonsil.
      The section was incubated with ab317685 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

      This data was developed using ab317685, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression: no staining on human cardiac muscle.
      The section was incubated with ab317685 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

      This data was developed using ab317685, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) Hela (Human cervix adenocarcinoma epithelial cell) cell pellet (B) A-549 (Human lung carcinoma epithelial cell) cell pellet tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) Hela cell pellet. Very weak staining on (B) A-549 cell pellet.
      The section was incubated with ab317685 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

      This data was developed using ab317685, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell) (Right) / 143B (human osteosarcoma cell) (Left) cells labelling SLC38A5 / SNAT5 with ab317685 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

      Low expression: 143B (PMID: 27129276). Gated on viable cells.

    • Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

      This data was developed using ab317685, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell) (Right) / A549 (human lung carcinoma epithelial cell) (Left) cells labelling SLC38A5 / SNAT5 with ab317685 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

      Low expression: A549. Gated on viable cells.

    • Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)
      Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free (ab317686)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317686？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317686 尚未被引用在任何文献中。

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