重组Anti-SLC38A5 / SNAT5抗体[EPR27109-86] (ab317685)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-1-anti-slc38a5snat5-antibody-epr27109-86-immunohistochemistry-human-pancreas.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27109-86] to SLC38A5 / SNAT5
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SLC38A5 / SNAT5抗体[EPR27109-86]
参阅全部 SLC38A5 / SNAT5 一抗 -
描述
兔单克隆抗体[EPR27109-86] to SLC38A5 / SNAT5 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, Flow Cytmore details
不适用于: ICC/IF,IP or WB -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human pancreas tissue, Human tonsil tissue, Hela cell pellet and A-549 cell pellet. Flow Cyt: HeLa and HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27109-86 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Flow Cyt |
1/50.
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| 说明 |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
1/50. |
靶标
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功能
Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium. -
组织特异性
Predominantly expressed in stomach, brain, liver, lung and intestinal tract. -
序列相似性
Belongs to the amino acid/polyamine transporter 2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 92745 Human
- Omim: 300649 Human
- SwissProt: Q8WUX1 Human
- Unigene: 195155 Human
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别名
- Amino acid transporter system N2 antibody
- JM 24 antibody
- JM24 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human pancreas (PMID: 37887353).
The section was incubated with ab317685 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-2-anti-slc38a5snat5-antibody-epr27109-86-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human tonsil.
The section was incubated with ab317685 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-3-anti-slc38a5snat5-antibody-epr27109-86-immunohistochemistry-human-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on human cardiac muscle.
The section was incubated with ab317685 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-4-anti-slc38a5snat5-antibody-epr27109-86-immunohistochemistry-a-hela-human-cerv.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)Immunohistochemical analysis of paraffin-embedded (A) Hela (Human cervix adenocarcinoma epithelial cell) cell pellet (B) A-549 (Human lung carcinoma epithelial cell) cell pellet tissue labeling SLC38A5 / SNAT5 with ab317685 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) Hela cell pellet. Very weak staining on (B) A-549 cell pellet.
The section was incubated with ab317685 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-5-anti-slc38a5snat5-antibody-epr27109-86-flow-cytometry-hela.jpg)
Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell) (Right) / 143B (human osteosarcoma cell) (Left) cells labelling SLC38A5 / SNAT5 with ab317685 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.
Low expression: 143B (PMID: 27129276). Gated on viable cells.
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![Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-6-anti-slc38a5snat5-antibody-epr27109-86-flow-cytometry-hepg2.jpg)
Flow Cytometry - Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell) (Right) / A549 (human lung carcinoma epithelial cell) (Left) cells labelling SLC38A5 / SNAT5 with ab317685 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.
Low expression: A549. Gated on viable cells.
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![Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)](https://www.abcam.cn/ps/products/317/ab317685/Images/ab317685-7-benefitsofrecombinantantibodies.jpg)
Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (ab317685)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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