AB228474

重组Anti-SLC34A2抗体[SP322] - N-terminal

Name: SLC34A2抗体[SP322] - N-terminal (ab228474)| Abcam中文官网
Brand: Abcam Limited
SKU: AB228474
Price: 1986 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-SLC34A2 antibody [SP322] - N-terminal

BOND RX™ Validated
20ul selling size
RabMAb
Advanced Validation
Recombinant
了解详情

(2 Reviews)

(2 Publications)

Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474) is a rabbit monoclonal antibody detecting SLC34A2 in Flow Cytometry (Intra), IHC-P, ICC/IF, mIHC. Suitable for Human.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency

查看别名

Sodium-dependent phosphate transport protein 2B, Sodium-phosphate transport protein 2B, Na(+)-dependent phosphate cotransporter 2B, NaPi3b, Sodium/phosphate cotransporter 2B, Solute carrier family 34 member 2, Na(+)/Pi cotransporter 2B, NaPi-2b, SLC34A2

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the luminal surface of human endometrial carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228474 for 10 mins at room temperature.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunocytochemistry/ Immunofluorescence analysis of NIH : OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labeling SLC34A2 with purified ab228474 at 1/100 (2.4 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228474 for 10 mins at room temperature.

Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Flow cytometry analysis of OvCAR-3 (human ovary adenocarcinoma) labeling SLC34A2 with purified ab228474 at 1/20 dilution (11.85 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-E Cadherin (ab256580, red; Opal™690), anti-SLC34A2 (ab238793, green; Opal™520) and anti-CD10 (ab255609, cyan; Opal™570) on human endometrium. Panel B : anti-CD10 stained on stromal cells. Panel C : anti-E Cadherin stained on glandular cells. Panel D : anti-SLC34A2 stained on apical membrane of glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab256580 at 1/3000 dilution (0.324 μg/ml) for 30mins, ab238793 at 1/1000 dilution (2.26 μg/ml) for 10mins and ab255609 at 1/1000 dilution (0.615 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This image was generated using ab238793, the same clone, but with a different buffer formulation.

不同偶联物与剂型 (4)

Carrier free

Anti-SLC34A2 antibody [SP322] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SLC34A2 - N-terminal antibody [SP322]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-SLC34A2 - N-terminal antibody [SP322]
578 PE

PE Anti-SLC34A2 antibody [SP322] - N-terminal

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

SP322

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, Flow Cyt (Intra), mIHC, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary antibody incubation for 10 minutes at room temperature.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human samples.

Collaboration
Anti-SLC34A2 [SP322] - N-terminal (ab228474) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [SP322] also available for your convenience: ab228474, Carrier free - ab238793, Alexa Fluor® 488 - ab315178, Alexa Fluor® 647 - ab315179, PE - ab322453

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

纯化说明

Purified from TCS by protein A/G.

存储溶液

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in actively transporting phosphate into cells via Na(+) cotransport.

See full target information SLC34A2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2021:3198555 PubMed34336552

2021

Evaluation of the Prognostic Value of Solute Carrier Family 34 Member 2 "SLC34A2" in Papillary Thyroid Carcinoma: An Immunohistochemical Study.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Adel Hakim,Rasha Mohamed Abd El Atti,Reham Mohamed Faheim,Hoda Hassan Abou Gabal

Cancer immunology, immunotherapy : CII 68:1621-1633 PubMed31549213

2019

The BRCA2 mutation status shapes the immune phenotype of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Jenzer,Peter Keß,Cathleen Nientiedt,Volker Endris,Maximilian Kippenberger,Jonas Leichsenring,Fabian Stögbauer,Josh Haimes,Skyler Mishkin,Brian Kudlow,Adam Kaczorowski,Stefanie Zschäbitz,Anna-Lena Volckmar,Holger Sültmann,Dirk Jäger,Anette Duensing,Peter Schirmacher,Markus Hohenfellner,Carsten Grüllich,Albrecht Stenzinger,Stefan Duensing

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SLC34A2抗体[SP322] - N-terminal

Anti-SLC34A2 antibody [SP322] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunocytochemistry/ Immunofluorescence - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Flow Cytometry (Intracellular) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)