重组Anti-SLC22A3/OCT3抗体[EPR6630] (ab124826)
![Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.cn/ps/products/124/ab124826/Images/ab124826-138768-anti-slc22a3-emt-antibody-epr6630-western-blot.jpg "Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6630] to SLC22A3/OCT3
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SLC22A3/OCT3抗体[EPR6630]
参阅全部 SLC22A3/OCT3 一抗 -
描述
兔单克隆抗体[EPR6630] to SLC22A3/OCT3 -
宿主
Rabbit -
特异性
We recommend using un-boiled sample to run WB tests. This antibody was found to have cross reactivity with SLC22A2 family member.
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经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human SLC22A3/OCT3 aa 300-400. The exact sequence is proprietary.
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阳性对照
- Human fetal kidney, Human muscle and Human placenta lysates; Human kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 4.75 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6630 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124826于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain. -
组织特异性
Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen. -
序列相似性
Belongs to the major facilitator superfamily. Organic cation transporter family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6581 Human
- Omim: 604842 Human
- SwissProt: O75751 Human
- Unigene: 567337 Human
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别名
- EMT antibody
- EMT organic cation transporter 3 antibody
- EMTH antibody
see all
图片
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Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)All lanes : Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human muscle tissue lysate
Lane 3 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.cn/ps/products/124/ab124826/Images/ab124826-208128-anti-slc22a3-emt-antibody-epr6630-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.cn/ps/products/124/ab124826/Images/ab124826-138767-anti-slc22a3-emt-antibody-epr6630-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)ab124826, at 1/100 dilution, staining SLC22A3/OCT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.cn/ps/products/124/ab124826/Images/ab124826-138766-anti-slc22a3-emt-antibody-epr6630.jpg)
OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) -
![Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.cn/ps/products/124/ab124826/Images/ab124826-5-benefits-of-recombinant-antibodies.png)
Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab124826 被引用在 11 文献中.
- Vachalova V et al. Functional reorganization of monoamine transport systems during villous trophoblast differentiation: evidence of distinct differences between primary human trophoblasts and BeWo cells. Reprod Biol Endocrinol 20:112 (2022). PubMed: 35927731
- Hau RK et al. Localization of Xenobiotic Transporters Expressed at the Human Blood-Testis Barrier. Drug Metab Dispos 50:770-780 (2022). PubMed: 35307651
- Cervenkova L et al. Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma. Sci Rep 9:19782 (2019). PubMed: 31874997
- Gu J et al. Upregulated OCT3 has the potential to improve the survival of colorectal cancer patients treated with (m)FOLFOX6 adjuvant chemotherapy. Int J Colorectal Dis 34:2151-2159 (2019). PubMed: 31732877
- Guttmann S et al. Organic cation transporter 3 mediates cisplatin and copper cross-resistance in hepatoma cells. Oncotarget 9:743-754 (2018). PubMed: 29416650