AB191315

Anti-SLC12A1/NKCC2 抗体 - N-terminal

Name: SLC12A1/NKCC2抗体- N-terminal (ab191315)| Abcam中文官网
Brand: Abcam Limited
SKU: AB191315
Price: 4514 CNY
Availability: InStock

Anti-SLC12A1/NKCC2 antibody - N-terminal

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(4 Publications)

Rabbit Polyclonal SLC12A1/NKCC2 antibody. N-terminal. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SLC12A1 aa 100-150.

查看别名

NKCC2, SLC12A1, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Kidney-specific Na-K-Cl symporter, Na-K-2Cl cotransporter 2, BSC1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemical analysis of paraffin-embedded section of rat kidney tissue, labelling SLC12A1/NKCC2 with ab191315 at 2 μg/ml.

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemical analysis of paraffin-embedded section of mouse kidney tissue, labelling SLC12A1/NKCC2 with ab191315 at 2 μg/ml.

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

IHC-P

applications

免疫原

Synthetic Peptide within Human SLC12A1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q13621

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.

Biological function summary

This transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.

Pathways

The SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.

Dysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed : 21321328). Electrically silent transporter system (By similarity).

See full target information SLC12A1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental nephrology 29:393-404 PubMed39666151

2024

Nephrotic syndrome induces the upregulation of cell proliferation-related genes in tubular cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Suzuki,Ryohei Kaseda,Yusuke Nakagawa,Hirofumi Watanabe,Tadashi Otsuka,Suguru Yamamoto,Yoshikatsu Kaneko,Shin Goto,Taiji Matsusaka,Ichiei Narita

FASEB journal : official publication of the Federa 33:5887-5902 PubMed30721632

2019

IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine Mohr,Jenny Voggel,Christina Vohlen,Katharina Dinger,Claudia Dafinger,Gregor Fink,Heike Göbel,Max C Liebau,Jörg Dötsch,Miguel A Alejandre Alcazar

Dose-response : a publication of International Hormesis Society 16:1559325818811522 PubMed30479587

2018

Sodium Dichloroacetate Pharmacological Effect as Related to Na-K-2Cl Cotransporter Inhibition in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jūratė Stanevičiūtė,Milda Juknevičienė,Jolita Palubinskienė,Ingrida Balnytė,Angelija Valančiūtė,Rūta Vosyliūtė,Kęstutis Sužiedėlis,Vaiva Lesauskaitė,Donatas Stakišaitis

BMC genomics 17:724 PubMed27613513

2016

A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle.

Applications

IHC-Fr

Species

Cow

Shinji Sasaki,Kiyotoshi Hasegawa,Tomoko Higashi,Yutaka Suzuki,Sumio Sugano,Yasuaki Yasuda,Yoshikazu Sugimoto

View all publications

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Anti-SLC12A1/NKCC2 抗体 - N-terminal

Anti-SLC12A1/NKCC2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)