AB181972

重组Anti-SLBP抗体[EPR12673]

Anti-SLBP antibody [EPR12673]

RabMAb
Recombinant
了解详情

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(8 Publications)

Rabbit Recombinant Monoclonal SLBP antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

查看别名

HBP, SLBP, Histone RNA hairpin-binding protein, Histone stem-loop-binding protein

6 Images

Flow Cytometry (Intracellular) - Anti-SLBP antibody [EPR12673] (AB181972)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SLBP antibody [EPR12673] (AB181972)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SLBP with Purified ab181972 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SLBP antibody [EPR12673] (AB181972)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling SLBP with ab181972 at 1/30 dilution (red) compared to aRabbit monoclonal IgG Isotype control (greeen), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

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Immunoprecipitation - Anti-SLBP antibody [EPR12673] (AB181972)

ab181972 (purified) at 1 : 20 dilution (1μg) immunoprecipitating SLBP in 293T whole cell lysate.
Lane 1 (input) : 293T (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab181972 & 293T whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181972 in 293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SLBP antibody [EPR12673] (ab181972)

Predicted band size: 31 kDa

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Supplier Data

Immunoprecipitation - Anti-SLBP antibody [EPR12673] (AB181972)

Western blot analysis of Jurkat cell lysate immunoprecipitated with ab181972 at 1/50 dilution (Lane 1). Lane 2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SLBP antibody [EPR12673] (ab181972)

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-SLBP antibody [EPR12673] (AB181972)

All lanes:

Western blot - Anti-SLBP antibody [EPR12673] (ab181972) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 31 kDa

Observed band size: 40 kDa

false

Unknown

Western blot - Anti-SLBP antibody [EPR12673] (AB181972)

All lanes:

Western blot - Anti-SLBP antibody [EPR12673] (ab181972) at 1/5000 dilution

All lanes:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-SLBP antibody [EPR12673] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12673

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1/50", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/30" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Stem-loop binding protein (SLBP) also known as histone stem-loop binding protein is critical in the processing and regulation of histone mRNA. SLBP is a 31 kDa protein and it is found in the nucleus where it facilitates histone mRNA processing specifically the maturation and stabilization of histone pre-mRNA. This protein binds to the stem-loop structure found at the 3’ end of replication-dependent histone mRNA which is essential for proper histone production during the S phase of the cell cycle.

Biological function summary

Stem-loop binding protein interacts with histone pre-mRNA processing machinery. It is not part of any large complex but plays an important role in association with the 3' end processing mechanism. By binding the stem-loop structure SLBP participates in ensuring the stability of histone mRNA ultimately influencing histone protein synthesis. The regulation of these mRNAs is important for DNA packaging and chromosome stability which are critical processes for normal cell function and division.

Pathways

SLBP is involved in the histone mRNA processing pathway which is pivotal for eukaryotic cell replication. This pathway includes notable participants like the U7 small nuclear ribonucleoprotein (snRNP). SLBP influences the balance of histone proteins in relation to DNA synthesis. The correct function in this pathway maintains the balance between DNA replication and nucleosome assembly which is related to the cell cycle and genome integrity maintenance.

Irregularities in SLBP function or expression link to certain cancers such as breast cancer where abnormal histone mRNA processing can occur. It is also associated with developmental disorders due to its role in cell division and DNA replication. In cases of cancer SLBP interacts with proteins like the p53 pathway where disruptions can lead to uncontrolled cell proliferation and tumor growth.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

RNA-binding protein involved in the histone pre-mRNA processing (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE) (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression (PubMed : 12588979, PubMed : 19155325, PubMed : 8957003, PubMed : 9049306). Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis (By similarity). Binds to the 5' side of the stem-loop structure of histone pre-mRNAs (By similarity).

See full target information SLBP

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Mechanobiology in medicine 3:100148 PubMed40995564

2025

Theta-shaking mitigates cognitive-emotional decline via subiculum and ventral septum metabolic plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Runhong Yao,Kouji Yamada,Hirohide Sawada,Takeshi Chihara,Naoki Aizu,Kazuhiro Nishii

Molecular cell 85:2487-2502.e12 PubMed40516529

2025

CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Ingham,Ignacio Alonso de Vega,Louise Morlot,William Gittens,Ivo A Hendriks,Ellen S Kakulidis,Raimundo Freire,Norman E Davey,Julien P Duxin,Michael Lund Nielsen,Niels Mailand

Journal of cell science 137: PubMed39140134

2024

Hominini-specific regulation of the cell cycle by stop codon readthrough of FEM1B.

Applications

Unspecified application

Species

Unspecified reactive species

Md Noor Akhtar,Anumeha Singh,Lekha E Manjunath,Dhruba Dey,Sangeetha Devi Kumar,Kirtana Vasu,Arpan Das,Sandeep M Eswarappa

Cell reports 43:114510 PubMed39018246

2024

Proteomic analysis reveals a PLK1-dependent G2/M degradation program and a role for AKAP2 in coordinating the mitotic cytoskeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan D Mouery,Kimberly Lukasik,Carolyn Hsu,Thomas Bonacci,Derek L Bolhuis,Xianxi Wang,C Allie Mills,E Drew Toomer,Owen G Canterbury,Kevin C Robertson,Timothy B Branigan,Nicholas G Brown,Laura E Herring,Stephanie L Gupton,Michael J Emanuele

Translational oncology 39:101837 PubMed37984255

2023

Dual role of CASP8AP2/FLASH in regulating epithelial-to-mesenchymal transition plasticity (EMP).

Applications

Unspecified application

Species

Unspecified reactive species

Madison Catalanotto,Joel Markus Vaz,Camille Abshire,Reneau Youngblood,Min Chu,Herbert Levine,Mohit Kumar Jolly,Ana-Maria Dragoi

International journal of molecular sciences 24: PubMed36614035

2022

HMGA1 Regulates the Expression of Replication-Dependent Histone Genes and Cell-Cycle in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Petrosino,Sabrina Pacor,Silvia Pegoraro,Virginia Anna Gazziero,Giulia Canarutto,Silvano Piazza,Guidalberto Manfioletti,Riccardo Sgarra

Toxicological sciences : an official journal of the Society of Toxicology 189:186-202 PubMed35929799

2022

Downregulation of Stem-Loop Binding Protein by Nicotine via α7-Nicotinic Acetylcholine Receptor and Its Role in Nicotine-Induced Cell Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Danqi Chen,Amna Raja,Gabriele Grunig,Judith Zelikoff,Chunyuan Jin

Cell death & disease 11:538 PubMed32678076

2020

The integrated stress response induces R-loops and hinders replication fork progression.

Applications

Unspecified application

Species

Unspecified reactive species

Josephine Ann Mun Yee Choo,Denise Schlösser,Valentina Manzini,Anna Magerhans,Matthias Dobbelstein

View all publications

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重组Anti-SLBP抗体[EPR12673]