Anti-SLAM / CD150抗体[IPO-3] (ab2604)
Key features and details
- Mouse monoclonal [IPO-3] to SLAM / CD150
- Suitable for: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SLAM / CD150抗体[IPO-3]
参阅全部 SLAM / CD150 一抗 -
描述
小鼠单克隆抗体[IPO-3] to SLAM / CD150 -
宿主
Mouse -
特异性
This antibody is specific for SLAM. -
经测试应用
适用于: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to SLAM/ CD150. Activated B cell line
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表位
The exact epitope recognised by this antibody has not been mapped. -
常规说明
A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells. -
克隆
单克隆 -
克隆编号
IPO-3 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use at an assay dependent concentration. PubMed: 21379338
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 21379338 |
靶标
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功能
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
组织特异性
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
翻译后修饰
Phosphorylated by FYN. -
细胞定位
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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数据库链接
- Entrez Gene: 6504 Human
- Omim: 603492 Human
- SwissProt: Q13291 Human
- Unigene: 523660 Human
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别名
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab2604 被引用在 6 文献中.
- Tang Q et al. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model. Methods Mol Biol 2164:75-85 (2020). PubMed: 32607885
- Borel F et al. Survival Advantage of Both Human Hepatocyte Xenografts and Genome-Edited Hepatocytes for Treatment of a-1 Antitrypsin Deficiency. Mol Ther 25:2477-2489 (2017). PubMed: 29032169
- Currier MG et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). PubMed: 27152417
- Mateo M et al. The measles virus hemagglutinin ß-propeller head ß4-ß5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule. J Virol 87:9208-16 (2013). PubMed: 23760251
- Avota E et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . PubMed: 21379338
- Noyce RS et al. Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus. PLoS Pathog 7:e1002240 (2011). PubMed: 21901103
