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Immunology Cell Type Markers CD Non-lineage

Anti-SLAM / CD150抗体[IPO-3] (ab2604)

  • Datasheet
Submit a review Q&A (1)References (6)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [IPO-3] to SLAM / CD150
  • Suitable for: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-SLAM / CD150抗体[IPO-3]
    参阅全部 SLAM / CD150 一抗
  • 描述

    小鼠单克隆抗体[IPO-3] to SLAM / CD150
  • 宿主

    Mouse
  • 特异性

    This antibody is specific for SLAM.
  • 经测试应用

    适用于: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to SLAM/ CD150. Activated B cell line

  • 表位

    The exact epitope recognised by this antibody has not been mapped.
  • 常规说明


    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
  • 克隆

    单克隆
  • 克隆编号

    IPO-3
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    unknown
  • 研究领域

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

相关产品

  • Alternative Versions

    • Anti-SLAM / CD150 antibody [IPO-3] - BSA and Azide free (ab183269)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human SLAM / CD150 protein (denatured) (ab139259)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2604于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration. PubMed: 21379338
说明
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration. PubMed: 21379338

靶标

  • 功能

    High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
  • 组织特异性

    Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
  • 序列相似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 结构域

    The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
  • 翻译后修饰

    Phosphorylated by FYN.
  • 细胞定位

    Cell membrane. Present on the surface of B-cells and T-cells.
  • Target information above from: UniProt accession Q13291 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6504 Human
    • Omim: 603492 Human
    • SwissProt: Q13291 Human
    • Unigene: 523660 Human
    • 别名

      • 4933415F16 antibody
      • AA177906 antibody
      • CD 150 antibody
      • CD150 antibody
      • CD150 antigen antibody
      • CDw150 antibody
      • Estm51 antibody
      • Ipo 3 antibody
      • IPO-3 antibody
      • Ipo3 antibody
      • MGC151472 antibody
      • MGC151476 antibody
      • OTTHUMP00000025670 antibody
      • OTTHUMP00000060252 antibody
      • RGD1560634 antibody
      • Signaling lymphocytic activation molecule antibody
      • Signaling lymphocytic activation molecule family member 1 antibody
      • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
      • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
      • Signaling lymphocytic activation molecule precursor antibody
      • Slaf1 antibody
      • SLAF1_HUMAN antibody
      • SLAM family, member 1 antibody
      • Slamf 1 antibody
      • SLAMF1 antibody
      • SLAMF1 protein antibody
      see all

    实验方案

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab2604?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2604 被引用在 6 文献中.

    • Tang Q  et al. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model. Methods Mol Biol 2164:75-85 (2020). PubMed: 32607885
    • Borel F  et al. Survival Advantage of Both Human Hepatocyte Xenografts and Genome-Edited Hepatocytes for Treatment of a-1 Antitrypsin Deficiency. Mol Ther 25:2477-2489 (2017). PubMed: 29032169
    • Currier MG  et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). PubMed: 27152417
    • Mateo M  et al. The measles virus hemagglutinin ß-propeller head ß4-ß5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule. J Virol 87:9208-16 (2013). PubMed: 23760251
    • Avota E  et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . PubMed: 21379338
    • Noyce RS  et al. Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus. PLoS Pathog 7:e1002240 (2011). PubMed: 21901103

    客户评价及客户问答

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    提交评价 提交问题

    Question

    Inquiry: I've often seen SLAM CD150 referred to as a 70-75 KDa protein including on this web-page. Where does this information come from? The human SLAM CD150 protein is much smaller than this at about 330 amino acids? Am I missing something?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2013

    Answer



    You are correct the calculated molecular weight of SLAM is ca 37 kDa.

    Modification can significantly increase the molecular weight of proteins. SLAM is a sialylated phosphoglycoprotein.

    The effect the glycosylation has on the molecular weight of SLAM is nicely demonstrated in the following publication:

    Castro., A.G., et al. (1999). J Immunol. 163(11):5860-5870 (also attached FYI)

    Therefore if the protein is modified we expect is to run at ca 50-80 kDa depending on the extent of the modification.

    Read More

    Anja Hoffmann

    Abcam Scientific Support

    回复于 Aug 08 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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