重组Anti-Skp1抗体[EPR3304] (ab76502)
![Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)](https://www.abcam.cn/ps/products/76/ab76502/Images/ab76502-91956-anti-skp1-antibody-epr3304-western-blot.jpg "Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3304] to Skp1
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Skp1抗体[EPR3304]
参阅全部 Skp1 一抗 -
描述
兔单克隆抗体[EPR3304] to Skp1 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra), ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Skp1 aa 100-200. The exact sequence is proprietary.
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阳性对照
- 293T, HeLa, PC-12 and NIH/3T3 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3304 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
|
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| Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 19 kDa. |
|
Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the SKP1 family. - Information by UniProt
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数据库链接
- Entrez Gene: 6500 Human
- Entrez Gene: 21402 Mouse
- Entrez Gene: 287280 Rat
- Omim: 601434 Human
- SwissProt: P63208 Human
- SwissProt: Q9WTX5 Mouse
- SwissProt: Q6PEC4 Rat
- Unigene: 171626 Human
see all -
别名
- Cyclin A/CDK2 associated p19 antibody
- Cyclin A/CDK2 associated protein p19 antibody
- Cyclin-A/CDK2-associated protein p19 antibody
see all
图片
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Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)All lanes : Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labeled IgG at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa -
![Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (ab76502)](https://www.abcam.cn/ps/products/76/ab76502/Images/ab76502-215907-anti-skp1-antibody-epr3304-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (ab76502)Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Anti-Skp1 antibody [EPR3304] (ab76502)](https://www.abcam.cn/ps/products/76/ab76502/Images/ab76502-5-benefits-of-recombinant-antibodies.png)
Anti-Skp1 antibody [EPR3304] (ab76502)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab76502 被引用在 7 文献中.
- Schaefer EJ et al. BCOR and BCORL1 Mutations Drive Epigenetic Reprogramming and Oncogenic Signaling by Unlinking PRC1.1 from Target Genes. Blood Cancer Discov 3:116-135 (2022). PubMed: 35015684
- Zeng J et al. A Hypoxia-Induced SCFFBXL1 E3 Ligase Ubiquitinates and Degrades the MEN1 Tumor Suppressor to Promote Colorectal Cancer Tumorigenesis. Cancer Res Treat 54:525-540 (2022). PubMed: 34237211
- Wang Y et al. FRK inhibits glioblastoma progression via phosphorylating YAP and inducing its ubiquitylation and degradation by Siah1. Neuro Oncol 24:2107-2120 (2022). PubMed: 35723276
- Xia S et al. TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance. Cell Mol Gastroenterol Hepatol 12:1121-1143 (2021). PubMed: 33962073
- Conde-Rubio MDC et al. The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases. Cell Death Discov 7:164 (2021). PubMed: 34226511
- Salehi-Tabar R et al. The Tumor Suppressor FBW7 and the Vitamin D Receptor Are Mutual Cofactors in Protein Turnover and Transcriptional Regulation. Mol Cancer Res 17:709-719 (2019). PubMed: 30606768
- Kainulainen M et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014). WB . PubMed: 24403578