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重组RabMAb

重组Anti-SIRT7抗体[EPR23811-15] - BSA and Azide free (ab275035)

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Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
  • Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23811-15] to SIRT7 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-SIRT7抗体[EPR23811-15] - BSA and Azide free
    参阅全部 SIRT7 一抗

  • 描述

    兔单克隆抗体[EPR23811-15] to SIRT7 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, IPmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Hepa1-6, Neuro-2a, NIH/3T3, RAW 264.7, C6, HeLa, HEK-293T, HepG2 and MCF7 whole cell lysates; Mouse spleen, skeletal muscle and liver tissue lysates; Ratskeletal muscle and liver tissue lysates. IHC-P: Mouse liver tissue; Rat spleen tissue. IP: HeLa and Neuro-2a whole cell lysates.

  • 常规说明

    ab275035 is the carrier-free version of ab259968.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab275035于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.

  • 序列相似性

    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.

  • 翻译后修饰

    Phosphorylated during mitosis.

  • 细胞定位

    Cytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • NAD dependent deacetylase sirtuin 7 antibody
    • NAD dependent protein deacetylase sirtuin 7 antibody
    • NAD-dependent protein deacetylase sirtuin-7 antibody
    • Regulatory protein SIR2 homolog 7 antibody
    • Silent mating type information regulation 2 homolog antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody
    • SIR2 L7 antibody
    • SIR2 like protein 7 antibody
    • Sir2 related protein type 7 antibody
    • SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody
    • SIR2-like protein 7 antibody
    • SIR2L 7 antibody
    • SIR2L7 antibody
    • SIR7_HUMAN antibody
    • SIRT 7 antibody
    • SIRT7 antibody
    • Sirtuin 7 antibody
    • Sirtuin type 7 antibody
    • Sirtuin7 antibody
    see all

图片

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse liver (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    SIRT7 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug

    Lane 2: ab259968 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 67 seconds.

     

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    All lanes : Anti-CD16+CD32 antibody [93] (ab269968) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat liver tissue lysate
    Lane 5 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Low expression: skeletal muscle (PMID: 16618798).

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression pattern of SIRT7 is consistent with what has been described in the literature(PMID: 24771643, 16618798).

  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    SIRT7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab259968 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat spleen (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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