Anti-SIRT6抗体(ab62739)
Key features and details
- Rabbit polyclonal to SIRT6
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SIRT6抗体
参阅全部 SIRT6 一抗 -
描述
兔多克隆抗体to SIRT6 -
宿主
Rabbit -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow
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免疫原
Synthetic peptide:
GLPEIFDPPEELERK
conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 19-33 of Human SIRT6 -
阳性对照
- ICC: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62739于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (8) |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)).
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| ICC |
Use a concentration of 2.5 µg/ml.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 2-4 ug/mL is recommended using whole extracts of mouse 3T3 cells. Use at 1-2 ug/mL with whole extracts of human U87 cells. Detects a band of approximately 37 kDa (predicted molecular weight: 39 (human), 37 mouse kDa (mouse)). |
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ICC
Use a concentration of 2.5 µg/ml. |
靶标
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功能
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. -
序列相似性
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain. -
细胞定位
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions. - Information by UniProt
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数据库链接
- Entrez Gene: 51548 Human
- Entrez Gene: 50721 Mouse
- Omim: 606211 Human
- SwissProt: Q8N6T7 Human
- SwissProt: P59941 Mouse
- Unigene: 423756 Human
- Unigene: 131825 Mouse
- Unigene: 25643 Mouse
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别名
- 2810449N18Rik antibody
- AI043036 antibody
- Mono ADP ribosyltransferase sirtuin 6 antibody
see all
图片
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Western blot - Anti-SIRT6 antibody (ab62739)Lane 1 : Anti-SIRT6 antibody (ab62739) at 0.5 µg/ml
Lane 2 : Anti-SIRT6 antibody (ab62739) at 1 µg/ml
Lanes 3-4 : Anti-SIRT6 antibody (ab62739) at 2 µg/ml
Lanes 1-3 : Mouse 3T3 whole cell extract
Lane 4 : Mouse 3T3 whole cell extract with immunizing peptide
Secondary
All lanes : Goat Anti-Rabbit IgG,
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-SIRT6 antibody (ab62739)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SIRT6 with ab62739. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 2.5 μg/mL Anti-SIRT6 antibody - ChIP Grade (ab62739). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate.
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Immunocytochemistry - Anti-SIRT6 antibody (ab62739)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of mouse primary hepatocytes labeling SIRT6 with ab62739 at 1/200 dilution. The cells were fixed with paraformaldehyde, followed by blocking with 3% BSA for 2 hours at 20°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 488 secondary antibody was used at 1/10000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (71)
ab62739 被引用在 71 文献中.
- Xu K et al. Decreased Enterobacteriaceae translocation due to gut microbiota remodeling mediates the alleviation of premature aging by a high-fat diet. Aging Cell 22:e13760 (2023). PubMed: 36567449
- Huang S et al. SIRT6 mediates MRTF-A deacetylation in vascular endothelial cells to antagonize oxLDL-induced ICAM-1 transcription. Cell Death Discov 8:96 (2022). PubMed: 35246513
- Sebastian C et al. A non-dividing cell population with high pyruvate dehydrogenase kinase activity regulates metabolic heterogeneity and tumorigenesis in the intestine. Nat Commun 13:1503 (2022). PubMed: 35314684
- Gandhi S et al. Mitotic H3K9ac is controlled by phase-specific activity of HDAC2, HDAC3, and SIRT1. Life Sci Alliance 5:N/A (2022). PubMed: 35981887
- Chen Z et al. Sirt6 deficiency contributes to mitochondrial fission and oxidative damage in podocytes via ROCK1-Drp1 signalling pathway. Cell Prolif 55:e13296 (2022). PubMed: 35842903