Anti-SIRT4抗体(ab124521)
Key features and details
- Rabbit polyclonal to SIRT4
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-SIRT4抗体
参阅全部 SIRT4 一抗 -
描述
兔多克隆抗体to SIRT4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide surrounding amino acid 72 of Rat SIRT4.
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阳性对照
- Rat kidney tissue lysate
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3224 SIRT4 Antibody. 3224-100 is the same size as the 100 µg size of ab124521.
The antibody solution should be gently mixed before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 69% PBS, 0.5% BSA, 30% Glycerol (glycerin, glycerine), 0.15% EDTA -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124521于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 32 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 32 kDa. |
靶标
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功能
Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion. -
组织特异性
Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus. -
序列相似性
Belongs to the sirtuin family. Class II subfamily.
Contains 1 deacetylase sirtuin-type domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
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别名
- MGC130046 antibody
- MGC130047 antibody
- MGC57437 antibody
see all
图片
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Western blot - Anti-SIRT4 antibody (ab124521)Anti-SIRT4 antibody (ab124521) at 4 µg/ml + Rat kidney tissue lysate
Predicted band size: 32 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab124521 被引用在 11 文献中.
- Zhang P et al. Paeoniflorin Enhances the Sensitivity of ER-Positive Breast Cancer Cells to Tamoxifen through Promoting Sirtuin 4. Evid Based Complement Alternat Med 2022:6730559 (2022). PubMed: 35154350
- Wei W et al. SIRT4 Is Highly Expressed in Retinal Müller Glial Cells. Front Neurosci 16:840443 (2022). PubMed: 35185463
- Zhang J et al. LncRNA DLEU2 regulates sirtuins and mitochondrial respiratory chain complex IV: a novel pathway in obesity and offspring's health. Int J Obes (Lond) 46:969-976 (2022). PubMed: 35058572
- Meng L et al. Dysregulation of the Sirt5/IDH2 axis contributes to sunitinib resistance in human renal cancer cells. FEBS Open Bio 11:921-931 (2021). PubMed: 33455080
- Thangaraj A et al. HIV TAT-mediated microglial senescence: Role of SIRT3-dependent mitochondrial oxidative stress. Redox Biol 40:101843 (2021). PubMed: 33385630