Anti-SIRT4抗体(ab10140)
Key features and details
- Goat polyclonal to SIRT4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SIRT4抗体
参阅全部 SIRT4 一抗 -
描述
山羊多克隆抗体to SIRT4 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide:
SRCGELLPLIDPC
, corresponding to C terminal amino acids 302-314 of Human SIRT4. -
阳性对照
- IHC: Human skeletal muscle and pancreas tissue WB: Human kidney and U937 lysate, HEK293 cell lysate
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常规说明
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10140于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended.
|
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended. |
靶标
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功能
Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion. -
组织特异性
Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus. -
序列相似性
Belongs to the sirtuin family. Class II subfamily.
Contains 1 deacetylase sirtuin-type domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 519328 Cow
- Entrez Gene: 23409 Human
- Entrez Gene: 75387 Mouse
- Entrez Gene: 100125349 Pig
- Entrez Gene: 304539 Rat
- Omim: 604482 Human
- SwissProt: Q1JQC6 Cow
- SwissProt: Q9Y6E7 Human
see all -
别名
- MGC130046 antibody
- MGC130047 antibody
- MGC57437 antibody
see all
图片
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Anti-SIRT4 antibody (ab10140) at 1 µg/ml + Human Kidney Lysate (RIPA buffer).35 ug total protein was loaded.
Predicted band size: 36 kDaab10140 staining (1
Incubation with the primary Ab was carried out for 1 hr at room temperature.µ g/ml) of Human Kidney lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab10140 被引用在 19 文献中.
- Wei W et al. SIRT4 Is Highly Expressed in Retinal Müller Glial Cells. Front Neurosci 16:840443 (2022). PubMed: 35185463
- Wang Y et al. SIRT4-Catalyzed Deacetylation of Axin1 Modulates the Wnt/β-Catenin Signaling Pathway. Front Oncol 12:872444 (2022). PubMed: 35707358
- Qin M et al. Autophagy in Rat Müller Glial Cells Is Modulated by the Sirtuin 4/AMPK/mTOR Pathway and Induces Apoptosis under Oxidative Stress. Cells 11:N/A (2022). PubMed: 36078054
- Xu L et al. Circular RNA OMA1 regulates the progression of breast cancer via modulation of the miR-1276/SIRT4 axis. Mol Med Rep 24:N/A (2021). PubMed: 34414449
- Du L et al. Loss of SIRT4 promotes the self-renewal of Breast Cancer Stem Cells. Theranostics 10:9458-9476 (2020). PubMed: 32863939