Anti-SIRT3抗体(ab118334)
Key features and details
- Goat polyclonal to SIRT3
- Suitable for: WB
- Reacts with: Mouse, Caenorhabditis elegans
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SIRT3抗体
参阅全部 SIRT3 一抗 -
描述
山羊多克隆抗体to SIRT3 -
宿主
Goat -
特异性
ab118334 is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Caenorhabditis elegans
预测可用于: Rat, Horse, Human, Pig
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免疫原
Synthetic peptide:
RASGIPASKLVEAH
with a Cysteine residue linker, corresponding to internal sequence amino acids 93-106 of Mouse SIRT3 (NP_071878.2), or amino acids 170-183 (NP_001171275.1). -
阳性对照
- Mouse heart lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab118334 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118334于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain. -
翻译后修饰
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 23410 Human
- Entrez Gene: 64384 Mouse
- Entrez Gene: 293615 Rat
- Omim: 604481 Human
- SwissProt: Q9NTG7 Human
- SwissProt: Q8R104 Mouse
- Unigene: 716456 Human
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别名
- hSIRT 3 antibody
- hSIRT3 antibody
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
see all
图片
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Western blot - Anti-SIRT3 antibody (ab118334)Anti-SIRT3 antibody (ab118334) at 0.3 µg/ml (for 1 hour) + Mouse Heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab118334 被引用在 12 文献中.
- Pezzotta A et al. Sirt3 deficiency promotes endothelial dysfunction and aggravates renal injury. PLoS One 18:e0291909 (2023). PubMed: 37816025
- Pezzotta A et al. Low Nephron Number Induced by Maternal Protein Restriction Is Prevented by Nicotinamide Riboside Supplementation Depending on Sirtuin 3 Activation. Cells 11:N/A (2022). PubMed: 36291179
- Perico L et al. Post-translational modifications by SIRT3 de-2-hydroxyisobutyrylase activity regulate glycolysis and enable nephrogenesis. Sci Rep 11:23580 (2021). PubMed: 34880332
- Bao J et al. circFAM160A2 Promotes Mitochondrial Stabilization and Apoptosis Reduction in Osteoarthritis Chondrocytes by Targeting miR-505-3p and SIRT3. Oxid Med Cell Longev 2021:5712280 (2021). PubMed: 34646424
- Castrejón-Téllez V et al. Effect of a Resveratrol/Quercetin Mixture on the Reversion of Hypertension Induced by a Short-Term Exposure to High Sucrose Levels Near Weaning and a Long-Term Exposure That Leads to Metabolic Syndrome in Rats. Int J Mol Sci 21:N/A (2020). PubMed: 32210194