Anti-SIRP alpha抗体(ab8120)
Key features and details
- Rabbit polyclonal to SIRP alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SIRP alpha抗体
参阅全部 SIRP alpha 一抗 -
描述
兔多克隆抗体to SIRP alpha -
宿主
Rabbit -
特异性
Recognizes SIRPa1, 2 and 3. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Peptide corresponding to amino acids 487 to 503 ofhuman SIRPa1 (1). The sequences of immunogenicpeptide differ from those of mouse, rat and bovine by oneamino acid (1-7).
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阳性对照
- THP-1 whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 75-110 kDa.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 75-110 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
组织特异性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
序列相似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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别名
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
图片
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Western blot - Anti-SIRP alpha antibody (ab8120)Anti-SIRP alpha antibody (ab8120) at 1/2000 dilution + THP-1 whole cell lysate
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Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (ab8120)ab8120 at 1µg/ml staining SIRP alpha in THP-1 cells by ICC/IF
实验方案
数据表及文件
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Datasheet download
文献 (11)
ab8120 被引用在 11 文献中.
- Shui M et al. Anomalous Levels of CD47/Signal Regulatory Protein Alpha in the Hippocampus Lead to Excess Microglial Engulfment in Mouse Model of Perioperative Neurocognitive Disorders. Front Neurosci 16:788675 (2022). PubMed: 35360151
- Zhou Z et al. Tumor-intrinsic SIRPA promotes sensitivity to checkpoint inhibition immunotherapy in melanoma. Cancer Cell 40:1324-1340.e8 (2022). PubMed: 36332624
- Ding X et al. Loss of microglial SIRPa promotes synaptic pruning in preclinical models of neurodegeneration. Nat Commun 12:2030 (2021). PubMed: 33795678
- Liu Y et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel) 6:422-433 (2020). PubMed: 33313063
- Li L et al. Signal regulatory protein a protects podocytes through promoting autophagic activity. JCI Insight 5:N/A (2019). PubMed: 30888336
