Anti-SIN1抗体(ab64188)
Key features and details
- Rabbit polyclonal to SIN1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SIN1抗体
参阅全部 SIN1 一抗 -
描述
兔多克隆抗体to SIN1 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human): 19 amino acid peptide from near the amino terminus of human SIN1, isoform 1 [NP_001006618].
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2.5 µg/ml.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. -
组织特异性
Ubiquitously expressed, with highest levels in heart and skeletal muscle. -
序列相似性
Belongs to the SIN1 family. -
细胞定位
Cell membrane. Cytoplasmic vesicle. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 79109 Human
- Omim: 610558 Human
- SwissProt: Q9BPZ7 Human
- Unigene: 495138 Human
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别名
- MAPKAP 1 antibody
- MAPKAP1 antibody
- MEKK2 interacting protein 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (ab64188)Ab64188 (2.5ug/ml) staining human SIN1 in human brain, cortex tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
数据表及文件
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Datasheet download
文献 (1)
ab64188 被引用在 1 文献中.
- Zhang C et al. Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling. Proc Natl Acad Sci U S A 109:1667-72 (2012). WB ; Mouse . PubMed: 22307628
