重组Anti-SIM2抗体[EPR7878] (ab131161)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7878] to SIM2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SIM2抗体[EPR7878]
参阅全部 SIM2 一抗 -
描述
兔单克隆抗体[EPR7878] to SIM2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2 whole cell lysate (ab7900); Human fetal forebrain and Human fetal brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7878 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131161于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa). |
靶标
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功能
Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 Single-minded C-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6493 Human
- Omim: 600892 Human
- SwissProt: Q14190 Human
- Unigene: 146186 Human
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别名
- bHLHe15 antibody
- Class E basic helix loop helix protein 15 antibody
- Class E basic helix-loop-helix protein 15 antibody
see all
图片
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Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted? -
All lanes : Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution
Lane 1 : Human fetal forebrain lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131161 被引用在 1 文献中.
- Qin B et al. Long non-coding RNA CASC15 facilitates esophageal squamous cell carcinoma tumorigenesis via decreasing SIM2 stability via FTO-mediated demethylation. Oncol Rep 45:1059-1071 (2021). PubMed: 33650646