AB280575

重组Anti-SIK2抗体[EPR22722-23] - BSA and Azide free

Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SIK2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse samples.

查看别名

Snf1lk2, Sik2, Serine/threonine-protein kinase SIK2, Salt-inducible kinase 2, Serine/threonine-protein kinase SNF1-like kinase 2, SIK-2

3 Images

Lab

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

This data was developed using ab245211, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lanes 1-2 : 59 seconds; Lane 3 : 3 minutes.

All lanes:

Western blot - Anti-SIK2 antibody [EPR22722-23] (<a href='/products/primary-antibodies/sik2-antibody-epr22722-23-ab245211'>ab245211</a>) at 1/1000 dilution

Lane 1:

Mouse P70 brown adipose tissue lysate at 20 µg

Lane 2:

Mouse P70 white adipose tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

Lab

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

This data was developed using ab245211, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 12624099)

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SIK2 antibody [EPR22722-23] (<a href='/products/primary-antibodies/sik2-antibody-epr22722-23-ab245211'>ab245211</a>) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

3T3-L1 differentiation for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

Lab

Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (AB280575)

This data was developed using ab245211, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody doesn't react with SIK1.

Exposure time : 3 seconds

All lanes:

Western blot - Anti-SIK2 antibody [EPR22722-23] (<a href='/products/primary-antibodies/sik2-antibody-epr22722-23-ab245211'>ab245211</a>) at 1/1000 dilution

Lane 1:

293T transfected with a DDDDK-tagged mouse SIK1(WT) expression vector, whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a DDDDK-tagged mouse SIK2(WT) expression vector, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-SIK2 antibody [EPR22722-23]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22722-23

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant fragment - Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab280575 is the carrier-free version of ab245211.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SIK2 also known as Salt-Inducible Kinase 2 is a protein kinase involved in various cellular processes. It has a molecular mass of approximately 94 kDa. SIK2 is expressed in many tissues with high levels found in adipose tissue liver and skeletal muscle. As a kinase it phosphorylates specific target proteins regulating their activity and influencing cellular mechanisms.

Biological function summary

SIK2 participates in energy metabolism and cellular response to external stress. It interacts with several co-factors to form active complexes facilitating its activity. This protein regulates lipid and carbohydrate metabolism therefore playing a significant role in maintaining cellular energy homeostasis. Additionally SIK2 has a function in controlling the inflammatory response providing a link between metabolism and immune function.

Pathways

SIK2 acts as an essential mediator in the AMPK signaling pathway. This pathway is important for energy balance within cells and is related to other kinases like AMPK and other SIK family members. SIK2 also contributes to the regulation of the cAMP signaling pathway which is linked to various physiological processes. Through these pathways SIK2 influences processes such as glucose uptake and fat oxidation and interacts with proteins like CREB and HDACs.

SIK2 has implications in conditions like obesity and type 2 diabetes. Dysregulation of SIK2 activity can lead to metabolic imbalance and insulin resistance. This kinase is also associated with cancer progression particularly in ovarian cancer where it influences cell proliferation. In these diseases SIK2's connection to proteins such as AMPK and CREB suggests a complex role in disease modulation and potential therapeutic targeting.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed : 12624099, PubMed : 16817901, PubMed : 29211348). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction (PubMed : 12624099). Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed : 16817901). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity. In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (By similarity). Also plays a role in thymic T-cell development (PubMed : 34732767).

See full target information Sik2

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SIK2抗体[EPR22722-23] - BSA and Azide free