Anti-SIK2抗体(ab53423)
Key features and details
- Rabbit polyclonal to SIK2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SIK2抗体
参阅全部 SIK2 一抗 -
描述
兔多克隆抗体to SIK2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human SIK2 aa 100-150. The exact sequence is proprietary.
Database link: NP_056006 -
阳性对照
- Human brain
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53423于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120, 25 kDa (predicted molecular weight: 104 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120, 25 kDa (predicted molecular weight: 104 kDa). |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23235 Human
- Omim: 608973 Human
- SwissProt: Q9H0K1 Human
- Unigene: 269128 Human
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别名
- DKFZp434K1115 antibody
- EC 2.7.11.1 antibody
- KIAA0781 antibody
see all
图片
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Western blot - Anti-SIK2 antibody (ab53423)All lanes : Anti-SIK2 antibody (ab53423) at 2 µg/ml
Lane 1 : Human brain
Lane 2 : Human brain with immunizing peptide
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
NB While the predicted molecular weight is 104kDa, both the bands at 120kDa and at 25kDa are blocked by immunizing peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK2 antibody (ab53423)IHC image of ab53423 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53423, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (4)
ab53423 被引用在 4 文献中.
- Shi X et al. SIK2 promotes ovarian cancer cell motility and metastasis by phosphorylating MYLK. Mol Oncol 16:2558-2574 (2022). PubMed: 35278271
- Liu Y et al. Biochemical purification uncovers mammalian sterile 3 (MST3) as a new protein kinase for multifunctional protein kinases AMPK and SIK3. J Biol Chem 298:101929 (2022). PubMed: 35413286
- Liu B et al. MYBL2-induced PITPNA-AS1 upregulates SIK2 to exert oncogenic function in triple-negative breast cancer through miR-520d-5p and DDX54. J Transl Med 19:333 (2021). PubMed: 34353336
- Weber GJ et al. Transcriptome alterations following developmental atrazine exposure in zebrafish are associated with disruption of neuroendocrine and reproductive system function, cell cycle, and carcinogenesis. Toxicol Sci 132:458-66 (2013). PubMed: 23358194
