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重组RabMAb

重组Anti-SIGLEC6抗体[EPR28608-365] - BSA and Azide free (ab317310)

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Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
  • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
  • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
  • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
  • Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28608-365] to SIGLEC6 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-SIGLEC6抗体[EPR28608-365] - BSA and Azide free
    参阅全部 SIGLEC6 一抗

  • 描述

    兔单克隆抗体[EPR28608-365] to SIGLEC6 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cytmore details
    不适用于: ICC/IF,IHC-P,IP or WB

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow Cyt: Human PBMC, Human PBMC, U-937 and Isotype cells.

  • 常规说明

    ab317310 is the carrier-free version of ab317309

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.2
    Constituent: 100% PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR28608-365

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317310于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.
说明
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IHC-P,IP or WB.

靶标

  • 功能

    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

  • 组织特异性

    Expressed at high levels in placenta (cyto- and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine.

  • 序列相似性

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 结构域

    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD327 antibody
      • CD33 antigen-like 1 antibody
      • CD33L antibody
      • CD33L1 antibody
      • CD33L2 antibody
      • CDw327 antibody
      • OB-BP1 antibody
      • OBBP1 antibody
      • Obesity-binding protein 1 antibody
      • Sialic acid-binding Ig-like lectin 6 antibody
      • SIGL6_HUMAN antibody
      • Siglec-6 antibody
      • SIGLEC6 antibody
      see all

    图片

    • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
      Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)

      This data was developed using ab317309, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling SIGLEC6 with ab317309 at 1/50 dilution (1ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD3 conjugated to Brilliant Violet 421.
      Gated on viable cells.

    • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
      Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)

      This data was developed using ab317309, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling SIGLEC6 with ab317309 at 1/50 dilution (1ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Cells were co-stained with anti-CD19 conjugated to Alexa Fluor® 647.
      Gated on viable cells.

    • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
      Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)

      This data was developed using ab317309, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of U-937 (human histiocytic lymphoma monocyte) (Right) / HepG2 (human hepatocellular carcinoma epithelial cell) (Left) cells labelling SIGLEC6 with ab317309 at 1/50 dilution (1ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Low expression: HepG2.
      Gated on viable cells.

    • Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
      Flow Cytometry - Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)

      This data was developed using ab317309, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Isotype (Left) / 293T cells transfected with a SIGLEC6 expression vector containing a GFP-tag (Middle) /293T cells transfected an empty vector containing a GFP-tag (Right) cells labelling SIGLEC6 with ab317309 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)
      Anti-SIGLEC6 antibody [EPR28608-365] - BSA and Azide free (ab317310)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317310？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317310 尚未被引用在任何文献中。

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