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重组RabMAb

重组Anti-SIGLEC10 + SIGLEC11抗体[EPR29036-83] - BSA and Azide free (ab317449)

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Western blot - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
  • Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR29036-83] to SIGLEC10 + SIGLEC11 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-SIGLEC10 + SIGLEC11抗体[EPR29036-83] - BSA and Azide free

  • 描述

    兔单克隆抗体[EPR29036-83] to SIGLEC10 + SIGLEC11 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    WB signal is only observed in tissue lysates.

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt (Intra),ICC/IF or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human tonsil and Human spleen lysates. IHC-P: Human spleen, Human tonsil, Human endometrial carcinoma, HEK-293T transfected with a SIGLEC10 expression vector containing a Myc-His tag. and HEK-293T transfected with a SIGLEC11 expression vector containing a Myc-His tag.

  • 常规说明

    ab317449 is the carrier-free version of ab317448.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317449于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

靶标

图片

  • Western blot - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Western blot - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    All lanes : Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] (ab317448) at 1/1000 dilution

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : Human spleen tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 76 kDa why is the actual band size different from the predicted?


    Exposure time: 103 seconds


    This data was developed using ab317448, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: human skeletal muscle (PMID:11733002).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

    This data was developed using ab317448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SIGLEC10 + SIGLEC11 with ab317448 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human spleen (PMID: 11358961; PMID: 11284738; PMID: 11733002). The section was incubated with ab317448 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

    This data was developed using ab317448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SIGLEC10 + SIGLEC11 with ab317448 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human tonsil. The section was incubated with ab317448 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

    This data was developed using ab317448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling SIGLEC10 + SIGLEC11 with ab317448 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human endometrial carcinoma. The section was incubated with ab317448 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

    This data was developed using ab317448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SIGLEC10 + SIGLEC11 with ab317448 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle. The section was incubated with ab317448 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

    This data was developed using ab317448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a SIGLEC10 expression vector containing a Myc-His tag. (B) HEK-293T transfected with a SIGLEC11 expression vector containing a Myc-His tag. (C) HEK-293T transfected with a SIGLEC16 expression vector containing a Myc-His tag. (D) HEK-293T transfected with a SIGLEC5 expression vector containing a Myc-His tag. (E) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling SIGLEC10 + SIGLEC11 with ab317448 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    This antibody recognizes both SIGLEC10 and SIGLEC11. Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a SIGLEC10 expression vector containing a Myc-His tag and (B) HEK-293T transfected with a SIGLEC11 expression vector containing a Myc-His tag, negative staining on (C) HEK-293T transfected with a SIGLEC16 expression vector containing a Myc-His tag, (D) HEK-293T transfected with a SIGLEC5 expression vector containing a Myc-His tag and (E) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab317448 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)
    Anti-SIGLEC10 + SIGLEC11 antibody [EPR29036-83] - BSA and Azide free (ab317449)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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