Anti-SIGLEC H抗体[440c] (ab106174)
Key features and details
- Rat monoclonal [440c] to SIGLEC H
- Suitable for: IHC-Fr, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG2b
概述
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产品名称
Anti-SIGLEC H抗体[440c]
参阅全部 SIGLEC H 一抗 -
描述
大鼠单克隆抗体[440c] to SIGLEC H -
宿主
Rat -
经测试应用
适用于: IHC-Fr, Flow Cyt, Inhibition Assaymore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse SIGLEC H. Purified bone-marrow derived mouse IPC
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常规说明
Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab106174 was 0.2 µm filtered prior to formulation. -
Primary antibody说明
Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion. -
克隆
单克隆 -
克隆编号
440c -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| Inhibition Assay |
Use at an assay dependent concentration.
(For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of SIGLEC H to be inactivated). |
| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
Inhibition Assay
Use at an assay dependent concentration. (For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of SIGLEC H to be inactivated). |
靶标
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相关性
SIGLEC H a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. -
细胞定位
Cell Membrane -
数据库链接
- Entrez Gene: 233274 Mouse
- SwissProt: Q3Y597 Mouse
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别名
- sialic acid binding Ig like lectin H antibody
- SIGLEC like protein antibody
- Siglech antibody
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab106174 被引用在 2 文献中.
- Blasius AL et al. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 107:2474-6 (2006). PubMed: 16293595
- Blasius A et al. A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 103:4201-6 (2004). PubMed: 14695235
