Anti-Sialoadhesin/CD169抗体[HSn 7D2] (ab18619)
![Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)](https://www.abcam.cn/ps/products/18/ab18619/Images/ab18619-291142-anti-sialoadhesin-antibody-hsn-7d2-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)")
Key features and details
- Mouse monoclonal [HSn 7D2] to Sialoadhesin/CD169
- Suitable for: WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Sialoadhesin/CD169抗体[HSn 7D2]
参阅全部 Sialoadhesin/CD169 一抗 -
描述
小鼠单克隆抗体[HSn 7D2] to Sialoadhesin/CD169 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Frmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Sialoadhesin/CD169.
-
表位
Within domains 1-4. -
阳性对照
- WB: Human spleen and human lymph node whole tissue lysate. IHC-Fr: Human liver normal tissue.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
HSn 7D2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18619于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml.
|
| IHC-Fr |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. |
|
IHC-Fr
Use a concentration of 5 µg/ml. |
靶标
-
功能
Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. -
组织特异性
Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 6614 Human
- Omim: 600751 Human
- SwissProt: Q9BZZ2 Human
- Unigene: 31869 Human
-
别名
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
see all
图片
-
Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)
IHC image of Sialoadhesin/CD169 staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with ab18619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
![Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)](https://www.abcam.cn/ps/products/18/ab18619/Images/ab18619-477993-anti-cd169-antibody-hsn-7d2-western-blot-human.jpg)
Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)All lanes : Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566) at 1 µg/ml
Lane 1 : Human spleen whole tissue lysate
Lane 2 : Human lymph node whole tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.
The membrane was then blocked for an hour using 2% BSA before being incubated with ab269566 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
This image was produced using ab269566, the same antibody clone but in a different PBS only formulation
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab18619 被引用在 9 文献中.
- Chen W et al. IL-1 receptor-associated kinase 1 participates in the modulation of the NLRP3 inflammasome by tumor-associated macrophages in hepatocellular carcinoma. J Gastrointest Oncol 13:1317-1329 (2022). PubMed: 35837195
- Li S et al. Siglec1 enhances inflammation through miR-1260-dependent degradation of I?Ba in COPD. Exp Mol Pathol 113:104398 (2020). PubMed: 32007531
- Murakami T et al. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun 9:2436 (2018). PubMed: 29934525
- Oliveira JJ et al. The plasma biomarker soluble SIGLEC-1 is associated with the type I interferon transcriptional signature, ethnic background and renal disease in systemic lupus erythematosus. Arthritis Res Ther 20:152 (2018). PubMed: 30053827
- Jans J et al. Siglec-1 inhibits RSV-induced interferon gamma production by adult T cells in contrast to newborn T cells. Eur J Immunol 48:621-631 (2018). PubMed: 29266251
- Cutler AJ et al. Capturing the systemic immune signature of a norovirus infection: an n-of-1 case study within a clinical trial. Wellcome Open Res 2:28 (2017). PubMed: 28815218
- Fujita E et al. Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages. Am J Pathol 177:1143-54 (2010). ICC/IF ; Human . PubMed: 20696778
- Hartnell A et al. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood 97:288-96 (2001). PubMed: 11133773
- Steiniger B et al. The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone. Immunology 92:307-16 (1997). PubMed: 9415041