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Anti-Sialoadhesin/CD169抗体[3D6.112] (ab53443)

Submit a review Q&A (2)References (6)
Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

    Key features and details

    • Rat monoclonal [3D6.112] to Sialoadhesin/CD169
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG2a

    概述

    • 产品名称

      Anti-Sialoadhesin/CD169抗体[3D6.112]
      参阅全部 Sialoadhesin/CD169 一抗

    • 描述

      大鼠单克隆抗体[3D6.112] to Sialoadhesin/CD169

    • 宿主

      Rat

    • 经测试应用

      适用于: IHC-Frmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • 常规说明

      Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 纯化说明

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

    • Primary antibody说明

      Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

    • 克隆

      单克隆

    • 克隆编号

      3D6.112

    • 同种型

      IgG2a

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab53443于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr
    1/50 - 1/100.
    说明
    IHC-Fr
    1/50 - 1/100.

    靶标

    • 功能

      Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.

    • 组织特异性

      Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

    • 序列相似性

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • 细胞定位

      Secreted and Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD169 antibody
        • CD169 antigen antibody
        • dJ1009E24.1 antibody
        • DKFZp667F058 antibody
        • FLJ00051 antibody
        • FLJ00055 antibody
        • FLJ00073 antibody
        • FLJ00411 antibody
        • FLJ32150 antibody
        • Sialic acid binding Ig like lectin 1 antibody
        • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
        • Sialic acid binding immunoglobulin like lectin 1 antibody
        • Sialic acid-binding Ig-like lectin 1 antibody
        • Sialoadhesin antibody
        • Siglec 1 antibody
        • Siglec-1 antibody
        • Siglec1 antibody
        • SN antibody
        • SN_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)
        Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)

        ab53443 staining Sialoadhesin/CD169 in mouse spleen by Immunohistochemistry (Frozen sections).

      文献 (6)

      发表研究结果有使用 ab53443？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab53443 被引用在 6 文献中.

      • Chen Q  et al. Resident macrophages restrain pathological adipose tissue remodeling and protect vascular integrity in obese mice. EMBO Rep 22:e52835 (2021). PubMed: 34196465
      • Zheng J  et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). PubMed: 29848296
      • Ibrahim MK  et al. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun 82:3098-112 (2014). PubMed: 24818662
      • Ibrahim MK  et al. The malnutrition-related increase in early visceralization of Leishmania donovani is associated with a reduced number of lymph node phagocytes and altered conduit system flow. PLoS Negl Trop Dis 7:e2329 (2013). Mouse . PubMed: 23967356
      • Xiong YS  et al. Contribution of monocytes Siglec-1 in stimulating T cells proliferation and activation in atherosclerosis. Atherosclerosis 224:58-65 (2012). PubMed: 22789514
      • Zollo M  et al. Targeting monocyte chemotactic protein-1 synthesis with bindarit induces tumor regression in prostate and breast cancer animal models. Clin Exp Metastasis 29:585-601 (2012). PubMed: 22484917

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      I was told that Abcam had CD169-biotin but I cant seem to find this on the list. I would like to use it for staining mouse spleen sections (immunohistochemistry). Do you have this product?

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      Answer

      Thank you for contacting us.

      Unfortunately, we only have the non-conjugated antibody or with a FITC conjugate.

      However, you could conjugate the antibody yourself with one of our biotin conjugation kits. Please see the following datasheet links:

      https://www.abcam.com/SialoadhesinCD169-antibody-3D6112-ab53443.html (or use the following: https://www.abcam.com/SialoadhesinCD169-antibody-3D6112-ab53443.html).

      https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html (or use the following: https://www.abcam.com/Biotin-type-A-Conjugation-Kit-ab102865.html).



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Dear Sir or Madam, can you please tell me if antibody ab53443 is directed against the N-terminus of the the target protein and if I can thus use it for the detection of only the N-terminal domain? --

      Read More
      Answer

      Thank you for your inquiry. The immunogen for this antibody was the full length protein from mouse. The epitope has not been mapped by us, or anyone else to our knowledge. Therefore I am unable to answer your question. We do not know where this antibody binds to within the protein. I am sorry I did not have a positive answer for you on this occasion and hope this information is nevertheless helpful.

      Read More

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