Anti-SIAH1抗体(ab2237)
Key features and details
- Goat polyclonal to SIAH1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SIAH1抗体
参阅全部 SIAH1 一抗 -
描述
山羊多克隆抗体to SIAH1 -
宿主
Goat -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cow, Dog -
免疫原
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阳性对照
- WB: Rat liver lysate, human liver lysate. ICC/IF- U2OS cells and HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 31 kDa.
1 hour primary incubation at room temperature is recommended for this product. |
ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 31 kDa. 1 hour primary incubation at room temperature is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. -
组织特异性
Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the SINA (Seven in absentia) family.
Contains 1 RING-type zinc finger.
Contains 1 SIAH-type zinc finger. -
结构域
The RING-type zinc finger domain is essential for ubiquitin ligase activity.
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family. -
翻译后修饰
Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2. -
细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 534655 Cow
- Entrez Gene: 6477 Human
- Entrez Gene: 20437 Mouse
- Entrez Gene: 140941 Rat
- Omim: 602212 Human
- SwissProt: Q8IUQ4 Human
- SwissProt: P61092 Mouse
- SwissProt: Q920M9 Rat
see all -
别名
- E3 ubiquitin-protein ligase SIAH1 antibody
- FLJ08065 antibody
- hSIAH1 antibody
see all
图片
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Anti-SIAH1 antibody (ab2237) at 1 mg/ml + Human liver lysate at 35 µg
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer was used.
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Anti-SIAH1 antibody (ab2237) at 1 µg/ml + Rat liver lysate at 35 µg
Predicted band size: 31 kDa
Observed band size: 30, 37 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer was used.
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Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized HepG2 (human liver hepatocellular carcinoma cell) cells labelling SIAH1 with primary anti-SIAH1 antibody (ab2237) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and cytoplasmic staining. The nuclear counterstain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody.
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Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U2OS (human bone osteosarcoma epithelial cell) cells labelling SIAH1 with primary anti-SIAH1 antibody (ab2237) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and cytoplasmic staining. The nuclear counterstain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody.
数据表及文件
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Datasheet download
文献 (36)
ab2237 被引用在 36 文献中.
- Lee B et al. Synthesis and discovery of the first potent proteolysis targeting chimaera (PROTAC) degrader of AIMP2-DX2 as a lung cancer drug. J Enzyme Inhib Med Chem 38:51-66 (2023). PubMed: 36305287
- Zhang W et al. Increased methylation of ZNF671 suppresses tumor progression by promoting MAPK6 transcription in laryngeal carcinoma. Int J Biol Sci 19:2443-2457 (2023). PubMed: 37215982
- Yoon H et al. Hydrogen Peroxide Inhibits Hepatitis B Virus Replication by Downregulating HBx Levels via Siah-1-Mediated Proteasomal Degradation in Human Hepatoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37686160
- Lin L et al. Reactive oxygen species-induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure. J Cell Mol Med 26:2417-2427 (2022). PubMed: 35261172
- Gao W et al. SIAH1 reverses chemoresistance in epithelial ovarian cancer via ubiquitination of YBX-1. Oncogenesis 11:13 (2022). PubMed: 35273154