Anti-SI抗体(ab224085)
Key features and details
- Rabbit polyclonal to SI
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SI抗体 -
描述
兔多克隆抗体to SI -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SI aa 1-200.
Database link: P14410 -
阳性对照
- WB: Human small intestine tissue lysate. IHC-P: Human small intestine tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224085于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 209 kDa.
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| 说明 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 209 kDa. |
靶标
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功能
Plays an important role in the final stage of carbohydrate digestion. -
组织特异性
Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. -
疾病相关
Defects in SI are the cause of congenital sucrase-isomaltase deficiency (CSID) [MIM:222900]; also known as disaccharide intolerance I. CSID is an autosomal recessive intestinal disorder that is clinically characterized by fermentative diarrhea, abdominal pain, and cramps upon ingestion of sugar. The symptoms are the consequence of absent or drastically reduced enzymatic activities of sucrase and isomaltase. The prevalence of CSID is 0.02 % in individuals of European descent and appears to be much higher in Greenland, Alaskan, and Canadian native people. CSID arises due to post-translational perturbations in the intracellular transport, polarized sorting, aberrant processing, and defective function of SI. -
序列相似性
Belongs to the glycosyl hydrolase 31 family.
Contains 2 P-type (trefoil) domains. -
翻译后修饰
The precursor is proteolytically cleaved when exposed to pancreatic proteases in the intestinal lumen.
Sulfated. -
细胞定位
Apical cell membrane. Brush border. - Information by UniProt
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数据库链接
- Entrez Gene: 6476 Human
- Omim: 609845 Human
- SwissProt: P14410 Human
- Unigene: 429596 Human
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形式
Sucrase-isomaltase is composed of 2 polypeptide chains of similar size linked together by noncovalent bonds. The polypeptide chains show a high degree of overlapping substrate specificity and are synthesized as 1 long polypeptide chain which, by means of pancreatic proteases, is later split into the 2 subunits. -
别名
- Isomaltase antibody
- MGC131621 antibody
- MGC131622 antibody
see all
图片
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Western blot - Anti-SI antibody (ab224085)ab224085 at 0.4 µg/ml + Human small intestine tissue lysate
Predicted band size: 209 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)Immunohistochemical analysis of human small intestine tissue labeling SI with ab224085 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)Immunohistochemical analysis of human small intestine tissue labeling SI with ab224085 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)Immunohistochemical analysis of human stomach tissue labeling SI with ab224085 at 1/200 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SI antibody (ab224085)Immunohistochemical analysis of human colon tissue labeling SI with ab224085 at 1/200 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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