Anti-SHROOM4抗体(ab121316)
Key features and details
- Rabbit polyclonal to SHROOM4
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SHROOM4抗体 -
描述
兔多克隆抗体to SHROOM4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 1170-1300 of Human SHROOM4.
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阳性对照
- Human cerebral cortex tissue; U 251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121316于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Probable regulator of cytoskeletal architecture that plays an important role in development. May regulate cellular and cytoskeletal architecture by modulating the spatial distribution of myosin II. -
组织特异性
Expressed in all fetal and adult tissues investigated. Expressed in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. In brain regions detected in cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe and putamen. The expression is strongest in the medulla and weakest in the cerebral cortex. -
疾病相关
Defects in SHROOM4 are the cause of mental retardation syndromic X-linked Stocco dos Santos type (SDSX) [MIM:300434]. A syndrome characterized by severe mental retardation with hyperactivity, aggressive behavior, delayed or no speech, and seizures. Additional features include congenital bilateral hip luxation, short stature, and kyphosis.
Note=A chromosomal aberration involving SHROOM4 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with FBXO25.
Note=A chromosomal aberration involving SHROOM4 is a cause of X-linked mental retardation (XLMR). Translocation t(X;19). -
序列相似性
Belongs to the Shroom family.
Contains 1 ASD2 domain.
Contains 1 PDZ (DHR) domain. -
细胞定位
Cytoplasm > cytoskeleton. Shows partial colocalization with the cytoplasmic pool of F-actin. - Information by UniProt
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数据库链接
- Entrez Gene: 57477 Human
- Omim: 300579 Human
- SwissProt: Q9ULL8 Human
- Unigene: 420541 Human
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别名
- Protein Shroom4 antibody
- RP11-119E20.1 antibody
- Second homolog of apical protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHROOM4 antibody (ab121316)ab121316, at 1/50 dilution, staining SHROOM4 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-SHROOM4 antibody (ab121316)ab1216316, at 4µg/ml, staining SHROOM4 in in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121316 尚未被引用在任何文献中。