Anti-SHP2抗体(ab9214)
Key features and details
- Goat polyclonal to SHP2
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SHP2抗体
参阅全部 SHP2 一抗 -
描述
山羊多克隆抗体to SHP2 -
宿主
Goat -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Rat, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human SHP2 aa 550 to the C-terminus (C terminal). (NP_001317366.1)
Database link: NP_002825.3 -
阳性对照
- WB: Human muscle lysate. ICC/IF: HeLa cells Flow Cyt (intra): A431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product. |
|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
靶标
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功能
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. -
组织特异性
Widely expressed, with highest levels in heart, brain, and skeletal muscle. -
疾病相关
Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
结构域
The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme. -
翻译后修饰
Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5781 Human
- Entrez Gene: 25622 Rat
- Omim: 176876 Human
- SwissProt: Q06124 Human
- SwissProt: P41499 Rat
- Unigene: 506852 Human
- Unigene: 98209 Rat
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别名
- BPTP3 antibody
- CFC antibody
- JMML antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SHP2 antibody (ab9214)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% triton-permeabilized HeLa cells staining SHP2 antibody with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. DAPI was used as a nuclear counterstain.
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody at 2ug/ml
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Flow Cytometry (Intracellular) - Anti-SHP2 antibody (ab9214)Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-permeabilized A431 cells (blue) staining SHP2 with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 1ug/ml. Primary incubation carried out for one hour.
IgG control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody (black).
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Western blot - Anti-SHP2 antibody (ab9214)Anti-SHP2 antibody (ab9214) at 2 µg/ml + Human muscle lysate at 35 µg
Predicted band size: 68 kDa -
Western blot - Anti-SHP2 antibody (ab9214)ab9214 staining (2µ g/ml) of Human Muscle lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab9214 被引用在 5 文献中.
- Ding Y et al. Tyrosine phosphatase SHP2 exacerbates psoriasis-like skin inflammation in mice via ERK5-dependent NETosis. MedComm (2020) 3:e120 (2022). PubMed: 35281792
- Sugimoto A et al. SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma. Sci Rep 10:2505 (2020). PubMed: 32054954
- Sugimoto A et al. SLAM family member 8 is involved in oncogenic KIT-mediated signalling in human mastocytosis. Exp Dermatol 27:641-646 (2018). PubMed: 29498772
- Liu HP et al. Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells. Cell Signal 23:487-96 (2011). WB ; Human . PubMed: 21070852
- Takagi K et al. Modulation of TNF-alpha gene expression by IFN-gamma and pamidronate in murine macrophages: regulation by STAT1-dependent pathways. J Immunol 174:1801-10 (2005). WB ; Mouse . PubMed: 15699106