AB79294

Anti-SHP1 (phospho Y564)抗体

Name: SHP1(磷酸化Y564)抗体 (ab79294)| Abcam中文官网
Brand: Abcam Limited
SKU: AB79294
Price: 4606 CNY
Availability: InStock

Anti-SHP1 (phospho Y564) antibody

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(1 Publication)

Rabbit Polyclonal SHP1 phospho Y564 antibody. Suitable for WB and reacts with African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PTPN6 phospho Y564.

查看别名

HCP, PTP1C, PTPN6, Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTP-1C

1 Images

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Western blot - Anti-SHP1 (phospho Y564) antibody (AB79294)

All lanes:

Western blot - Anti-SHP1 (phospho Y564) antibody (ab79294) at 1/1000 dilution

Lane 1:

extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins) at 30 µg

Lane 2:

extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins) at 30 µg with immunising peptide

Predicted band size: 68 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

African green monkey

应用

applications

免疫原

Synthetic Peptide within Human PTPN6 phospho Y564. The exact immunogen used to generate this antibody is proprietary information.

P29350

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab79294 is purified from rabbit antiserum by chromatography using an epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

存储溶液

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SHP1 also known as PTPN6 belongs to the family of protein tyrosine phosphatases and has a protein mass of approximately 68 kDa. It plays a critical role in cell signaling by dephosphorylating specific targets leading to the modulation of signal transduction pathways. SHP1 is ubiquitously expressed with high levels observed in hematopoietic cells suggesting a specialized function in the immune system.

Biological function summary

SHP1 influences cell proliferation survival and differentiation by acting as a negative regulator of signaling pathways. It is not a part of a larger protein complex but it associates transiently with different receptors and substrates. It plays an important role in the immune response by regulating cytokine signaling which is important for maintaining the balance between immune activation and tolerance.

Pathways

SHP1 significantly impacts the JAK/STAT and B cell receptor signaling pathways. In the JAK/STAT pathway SHP1 interacts with JAK kinases contributing to the downregulation of cytokine signaling. In B cell receptor signaling it associates with Syk influencing immune cell activation and function.

SHP1 has a role in autoimmune diseases such as rheumatoid arthritis and certain types of leukemia. In rheumatoid arthritis defective regulation by SHP1 can lead to excessive inflammation while in leukemia its altered activity impacts cell growth and survival. It interacts with proteins such as STATs and SOCS which are important in these pathologies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Tyrosine phosphatase enzyme that plays important roles in controlling immune signaling pathways and fundamental physiological processes such as hematopoiesis (PubMed : 14739280, PubMed : 29925997). Dephosphorylates and negatively regulate several receptor tyrosine kinases (RTKs) such as EGFR, PDGFR and FGFR, thereby modulating their signaling activities (PubMed : 21258366, PubMed : 9733788). When recruited to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors such as immunoglobulin-like transcript 2/LILRB1, programmed cell death protein 1/PDCD1, CD3D, CD22, CLEC12A and other receptors involved in immune regulation, initiates their dephosphorylation and subsequently inhibits downstream signaling events (PubMed : 11907092, PubMed : 14739280, PubMed : 37932456, PubMed : 38166031). Modulates the signaling of several cytokine receptors including IL-4 receptor (PubMed : 9065461). Additionally, targets multiple cytoplasmic signaling molecules including STING1, LCK or STAT1 among others involved in diverse cellular processes including modulation of T-cell activation or cGAS-STING signaling (PubMed : 34811497, PubMed : 38532423). Within the nucleus, negatively regulates the activity of some transcription factors such as NFAT5 via direct dephosphorylation. Also acts as a key transcriptional regulator of hepatic gluconeogenesis by controlling recruitment of RNA polymerase II to the PCK1 promoter together with STAT5A (PubMed : 37595871).

See full target information PTPN6 phospho Y564

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