Anti-SHP1 (phospho Y536)抗体(ab51171)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y536)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SHP1 (phospho Y536)抗体
参阅全部 SHP1 一抗 -
描述
兔多克隆抗体to SHP1 (phospho Y536) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human SHP1 aa 500-600 (phospho Y536).
Database link: P29350 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab51171 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/300 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 68 kDa).
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/300 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 68 kDa). |
靶标
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功能
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
组织特异性
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰
Phosphorylated on serine and tyrosine residues. -
细胞定位
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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数据库链接
- Entrez Gene: 5777 Human
- Entrez Gene: 15170 Mouse
- Omim: 176883 Human
- SwissProt: P29350 Human
- SwissProt: P29351 Mouse
- Unigene: 63489 Human
- Unigene: 271799 Mouse
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别名
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y536) antibody (ab51171)ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes) Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab51171 被引用在 11 文献中.
- Li Y et al. WASp Is Crucial for the Unique Architecture of the Immunological Synapse in Germinal Center B-Cells. Front Cell Dev Biol 9:646077 (2021). PubMed: 34195186
- Akhtar MN et al. Runx proteins mediate protective immunity against Leishmania donovani infection by promoting CD40 expression on dendritic cells. PLoS Pathog 16:e1009136 (2020). PubMed: 33370418
- Jiang L et al. SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-ß signaling. J Exp Med 215:1337-1347 (2018). PubMed: 29669741
- Yao D et al. CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway. Int J Clin Exp Pathol 11:5612-5621 (2018). PubMed: 31949648
- Savola P et al. Somatic mutations in clonally expanded cytotoxic T lymphocytes in patients with newly diagnosed rheumatoid arthritis. Nat Commun 8:15869 (2017). PubMed: 28635960