Anti-SHP1 (phospho Y536)抗体(ab131500)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y536)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SHP1 (phospho Y536)抗体
参阅全部 SHP1 一抗 -
描述
兔多克隆抗体to SHP1 (phospho Y536) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SHP1 (phospho Y536) conjugated to keyhole limpet haemocyanin.
Database link: NP_002822.2 -
阳性对照
- HeLa cells; Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131500 was purified by affinity chromatography using epitope specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131500于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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说明 |
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IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
组织特异性
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰
Phosphorylated on serine and tyrosine residues. -
细胞定位
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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数据库链接
- Entrez Gene: 5777 Human
- Omim: 176883 Human
- SwissProt: P29350 Human
- Unigene: 63489 Human
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别名
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
图片
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHP1 (phospho Y536) with ab131500 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y536) antibody (ab131500)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHP1 (phospho Y536) with ab131500 at 1/50 dilution (left) or ab131500 preincubated with blocking peptide (right).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab131500 被引用在 4 文献中.
- Flores AM et al. Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis. Nat Nanotechnol 15:154-161 (2020). PubMed: 31988506
- Gerlach BD et al. Resolvin D1 promotes the targeting and clearance of necroptotic cells. Cell Death Differ 27:525-539 (2020). PubMed: 31222041
- Kojima Y et al. Proefferocytic Therapy Promotes Transforming Growth Factor-ß Signaling and Prevents Aneurysm Formation. Circulation 137:750-753 (2018). PubMed: 29440201
- Kojima Y et al. CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis. Nature 536:86-90 (2016). PubMed: 27437576