Anti-SHMT2/SHMT抗体- C-terminal (ab180786)
Key features and details
- Rabbit polyclonal to SHMT2/SHMT - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SHMT2/SHMT抗体- C-terminal
参阅全部 SHMT2/SHMT 一抗 -
描述
兔多克隆抗体to SHMT2/SHMT - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human SHMT2/SHMT aa 265-504 (C terminal).
Database link: P34897 -
阳性对照
- WB: HeLa, OVCAR3 and 22Rv1 cell lysates, mouse liver and kidney tissue lysates; IHC: Human liver cancer tissue, mouse and rat spleen tissues; ICC/ IF: L929 and U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180786于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
|
|
| ICC/IF | (1) |
1/50 - 1/200.
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 56 kDa. |
|
ICC/IF
1/50 - 1/200. |
靶标
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功能
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. -
通路
One-carbon metabolism; tetrahydrofolate interconversion. -
序列相似性
Belongs to the SHMT family. -
细胞定位
Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6472 Human
- Entrez Gene: 108037 Mouse
- Entrez Gene: 299857 Rat
- Omim: 138450 Human
- SwissProt: P34897 Human
- Unigene: 741179 Human
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别名
- Epididymis secretory sperm binding protein Li 51e antibody
- GLY A+ antibody
- GLYA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Paraffin-embedded human liver cancer tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Immunofluorescence staining of L929 cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)All lanes : Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : 22Rv1 cell lysate
Lane 4 : Mouse liver tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 56 kDaBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Paraffin-embedded mouse spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Immunofluorescence staining of U-2 OS cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Paraffin-embedded rat spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab180786 被引用在 8 文献中.
- Yang C et al. Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment. Cell Death Dis 14:525 (2023). PubMed: 37582769
- Thompson LL et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628
- Yang Y et al. RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer. Cancer Cell Int 20:587 (2020). PubMed: 33372599
- Qi C et al. Circ_0072995 Promotes Cell Carcinogenesis via Up-Regulating miR-149-5p-Mediated SHMT2 in Breast Cancer. Cancer Manag Res 12:11169-11181 (2020). PubMed: 33173349
- Xu T et al. MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2. Am J Cancer Res 9:250-269 (2019). PubMed: 30906627
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746
- Wu M et al. Overexpression of mitochondrial serine hydroxyl-methyltransferase 2 is associated with poor prognosis and promotes cell proliferation and invasion in gliomas. Onco Targets Ther 10:3781-3788 (2017). PubMed: 28794642
- Yin K Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade. Onco Targets Ther 8:1069-74 (2015). IHC-P ; Human . PubMed: 25999742