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AB88664

Anti-SHMT2/SHMT抗体

Anti-SHMT2/SHMT antibody

4

(2 Reviews)

|

(1 Publication)

Mouse Polyclonal SHMT2/SHMT antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Serine hydroxymethyltransferase, mitochondrial.

查看别名

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB88664)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB88664)

ab88664, at 10 μg/ml, staining SHMT2/SHMT in Hela cells by Immunofluorescence.

Western blot - Anti-SHMT2/SHMT antibody (AB88664)
  • WB

Unknown

Western blot - Anti-SHMT2/SHMT antibody (AB88664)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab88664) at 1 µg/mL

All lanes:

human spleen tissue lysate at 50 µg

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human Serine hydroxymethyltransferase, mitochondrial.

P34897

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替代产品

Primary Antibodies

AB155230

Anti-SHMT2/SHMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB155230)

  • 4
  • 1
Primary Antibodies

AB224428

Anti-SHMT2/SHMT antibody

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

  • 0
  • 0
Primary Antibodies

AB224427

Anti-SHMT2/SHMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224427)

  • 0
  • 0
Primary Antibodies

AB180786

Anti-SHMT2/SHMT antibody - C-terminal

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (AB180786)

  • 5
  • 2
Primary Antibodies

AB240606

Anti-SHMT2/SHMT antibody

Immunoprecipitation - Anti-SHMT2/SHMT antibody (AB240606)

  • 0
  • 0
Primary Antibodies

AB233430

Anti-SHMT2/SHMT antibody

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB233430)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB237704

Anti-SLC1A5/ASCT2 antibody [CAL33]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] (AB237704)

  • 0
  • 0
Primary Antibodies

AB88872

Anti-RPLP0 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody (AB88872)

  • 3
  • 2
Primary Antibodies

AB186130

Anti-SHMT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT1 antibody (AB186130)

  • 0
  • 0
Primary Antibodies

AB221925

Anti-MTHFD1L antibody

Immunocytochemistry/ Immunofluorescence - Anti-MTHFD1L antibody (AB221925)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).
See full target information Serine hydroxymethyltransferase, mitochondrial
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 36:2489-2500 PubMed27666119

2016

Prognostic and therapeutic value of mitochondrial serine hydroxyl-methyltransferase 2 as a breast cancer biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Lahong Zhang,Zhaojun Chen,Dan Xue,Qi Zhang,Xiyong Liu,Frank Luh,Liquan Hong,Hang Zhang,Feng Pan,Yuhua Liu,Peiguo Chu,Shu Zheng,Guoqiang Lou,Yun Yen
View all publications
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